Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPM17_RS25540 Genome accession   NZ_CP046872
Coordinates   5616759..5618021 (-) Length   420 a.a.
NCBI ID   WP_080637233.1    Uniprot ID   -
Organism   Pseudomonas putida strain MX-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5611759..5623021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPM17_RS25525 (GPM17_25430) - 5613425..5615026 (-) 1602 WP_003249151.1 SPOR domain-containing protein -
  GPM17_RS25530 (GPM17_25435) aroB 5615042..5616139 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  GPM17_RS25535 (GPM17_25440) aroK 5616236..5616754 (-) 519 WP_003249147.1 shikimate kinase AroK -
  GPM17_RS25540 (GPM17_25445) pilQ 5616759..5618021 (-) 1263 WP_080637233.1 type IV pilus secretin PilQ Machinery gene
  GPM17_RS25545 (GPM17_25450) - 5617945..5618946 (-) 1002 WP_185280402.1 pilus assembly protein PilP -
  GPM17_RS25550 (GPM17_25455) - 5618958..5619494 (-) 537 WP_098089508.1 PilN domain-containing protein -
  GPM17_RS25555 (GPM17_25460) pilM 5619491..5620372 (-) 882 WP_098089510.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45670.35 Da        Isoelectric Point: 4.9839

>NTDB_id=409055 GPM17_RS25540 WP_080637233.1 5616759..5618021(-) (pilQ) [Pseudomonas putida strain MX-2]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAEIRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=409055 GPM17_RS25540 WP_080637233.1 5616759..5618021(-) (pilQ) [Pseudomonas putida strain MX-2]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAGCTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATACGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGAGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.617
  comQ Acinetobacter baylyi ADP1

43.111

100

0.462
  pilQ Acinetobacter baumannii strain A118

41.215

100

0.452
  pilQ Acinetobacter baumannii D1279779

41.215

100

0.452
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

41.244

100

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.651

98.81

0.421
  pilQ Vibrio cholerae strain A1552

42.651

98.81

0.421

Multiple sequence alignment