Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H0X93_RS20465 Genome accession   NZ_CP059125
Coordinates   4220298..4220975 (+) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain EC45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4215298..4225975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0X93_RS20430 (H0X93_20360) gspG 4215778..4216215 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  H0X93_RS20435 (H0X93_20365) gspH 4216223..4216732 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  H0X93_RS20440 (H0X93_20370) gspI 4216729..4217106 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  H0X93_RS20445 (H0X93_20375) gspJ 4217099..4217686 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  H0X93_RS20450 (H0X93_20380) gspK 4217679..4218662 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  H0X93_RS20455 (H0X93_20385) gspL 4218677..4219840 (+) 1164 WP_001115230.1 type II secretion system protein GspL -
  H0X93_RS20460 (H0X93_20390) gspM 4219837..4220298 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  H0X93_RS20465 (H0X93_20395) pilD 4220298..4220975 (+) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  H0X93_RS20470 (H0X93_20400) bfr 4221004..4221480 (-) 477 WP_000675504.1 bacterioferritin -
  H0X93_RS20475 (H0X93_20405) bfd 4221552..4221746 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H0X93_RS20480 (H0X93_20410) chiA 4221915..4224608 (-) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=408707 H0X93_RS20465 WP_001377644.1 4220298..4220975(+) (pilD) [Escherichia coli strain EC45]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=408707 H0X93_RS20465 WP_001377644.1 4220298..4220975(+) (pilD) [Escherichia coli strain EC45]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373

Multiple sequence alignment