Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY55_RS09665 Genome accession   NZ_CP046831
Coordinates   124791..125807 (+) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus strain 2012AW-0224     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119791..130807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY55_RS09650 - 120215..120943 (-) 729 WP_015297397.1 redoxin family protein -
  GPY55_RS09655 oxyR 121114..122022 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GPY55_RS09660 - 122078..124648 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  GPY55_RS09665 pilM 124791..125807 (+) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  GPY55_RS09670 pilN 125791..126369 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  GPY55_RS09675 pilO 126362..126955 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY55_RS09680 pilP 126945..127460 (+) 516 WP_025517730.1 pilus assembly protein PilP Machinery gene
  GPY55_RS09685 pilQ 127492..129240 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY55_RS09690 aroK 129429..129947 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=408530 GPY55_RS09665 WP_005496568.1 124791..125807(+) (pilM) [Vibrio parahaemolyticus strain 2012AW-0224]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=408530 GPY55_RS09665 WP_005496568.1 124791..125807(+) (pilM) [Vibrio parahaemolyticus strain 2012AW-0224]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment