Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY06_RS11260 Genome accession   NZ_CP046820
Coordinates   1202762..1203778 (-) Length   338 a.a.
NCBI ID   WP_158120071.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197762..1208778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY06_RS11235 aroK 1198651..1199175 (-) 525 WP_000818614.1 shikimate kinase AroK -
  GPY06_RS11240 pilQ 1199362..1201077 (-) 1716 WP_167517861.1 type IV pilus secretin PilQ Machinery gene
  GPY06_RS11245 pilP 1201103..1201621 (-) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  GPY06_RS11250 pilO 1201611..1202201 (-) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY06_RS11255 pilN 1202194..1202772 (-) 579 WP_167517862.1 PilN domain-containing protein Machinery gene
  GPY06_RS11260 pilM 1202762..1203778 (-) 1017 WP_158120071.1 type IV pilus assembly protein PilM Machinery gene
  GPY06_RS11265 - 1203920..1206397 (+) 2478 WP_167517863.1 penicillin-binding protein 1A -
  GPY06_RS11270 oxyR 1206494..1207387 (-) 894 WP_055035148.1 DNA-binding transcriptional regulator OxyR -
  GPY06_RS11275 - 1207527..1208258 (+) 732 WP_095475740.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37558.33 Da        Isoelectric Point: 7.0270

>NTDB_id=408362 GPY06_RS11260 WP_158120071.1 1202762..1203778(-) (pilM) [Vibrio metoecus strain 2011V-1015]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSQKVAIAIPDNA
VISKVLQIDSDLELREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNLARSSTTTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRADWMLIDIGHTQSSLCLDFPDKMPFYKDVPLGARLVDLDSASPSILNPPQLQDSSQR
FINELVDKVARQIQLFTSVHGTQSLSGLWLSGGGAAMEGLPEALSQRLSLPCEVLNPFALFKMQVSKRKHQVVDGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=408362 GPY06_RS11260 WP_158120071.1 1202762..1203778(-) (pilM) [Vibrio metoecus strain 2011V-1015]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCCGTGGTGCTCAAACCTATGGGCGACAC
CTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTACCCCTATTTAGTCAGAAAGTCGCGATTGCAATCCCGGATAACGCG
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCTGCGAGAGCAGGAGTTCGCTATTTATCAGGCTTTTTCCCA
CCAATCTCCATTTCCCGTTGAAGATCTTAGCCTTGATTTCGTCAAAGTGGCGGAAAAAAATCTCGCACGCAGTAGTACGA
CGACGTTTCAAGTCTATGCCACCAAGAAAGAAGTGGTTGAAAGCCGCTTACAAGCCAGTAAAAAAGCAGGTTTTGAGCCG
ATCTTAATGGATGTGCAAGTGCACAGTTTGTTGCATCTATGGCAGTTGGCTAGCCGCGCGTATCGCCGTGCGGACTGGAT
GCTGATTGATATTGGACACACACAAAGCTCTTTGTGTTTGGACTTTCCAGACAAAATGCCTTTTTACAAAGATGTGCCAC
TAGGTGCTCGATTGGTCGATTTAGACTCTGCCAGCCCATCCATACTCAATCCGCCTCAATTGCAGGACTCATCTCAGCGT
TTTATCAATGAATTGGTGGATAAAGTGGCGCGTCAAATCCAACTGTTTACGTCGGTGCATGGAACACAAAGCCTCAGTGG
CTTATGGCTATCAGGCGGTGGTGCGGCGATGGAGGGATTGCCAGAAGCGTTGTCCCAGCGTTTATCTTTGCCTTGTGAAG
TGCTGAATCCTTTTGCGTTGTTCAAAATGCAGGTCTCAAAACGTAAACACCAAGTGGTTGATGGTCAGCGTTTTAGCACT
GCAGCAGGGCTCGCTTTGCGTGGTTTAGCATGGCTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-200)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

84.32

100

0.843
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment