Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX92_RS10130 Genome accession   NZ_CP046802
Coordinates   1250520..1251542 (+) Length   340 a.a.
NCBI ID   WP_078926721.1    Uniprot ID   A0A1T4QY68
Organism   Vibrio cincinnatiensis strain 2070-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1245520..1256542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX92_RS10110 - 1245719..1245988 (-) 270 WP_158117084.1 DUF3624 domain-containing protein -
  GPX92_RS10115 - 1246084..1246815 (-) 732 WP_078926724.1 glutathione peroxidase -
  GPX92_RS10120 oxyR 1246961..1247851 (+) 891 WP_078926723.1 DNA-binding transcriptional regulator OxyR -
  GPX92_RS10125 - 1247901..1250378 (-) 2478 WP_158117085.1 penicillin-binding protein 1A -
  GPX92_RS10130 pilM 1250520..1251542 (+) 1023 WP_078926721.1 type IV pilus assembly protein PilM Machinery gene
  GPX92_RS10135 pilN 1251532..1252125 (+) 594 WP_078926720.1 PilN domain-containing protein Machinery gene
  GPX92_RS10140 pilO 1252118..1252708 (+) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX92_RS10145 pilP 1252698..1253216 (+) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX92_RS10150 pilQ 1253241..1254962 (+) 1722 WP_078926717.1 type IV pilus secretin PilQ Machinery gene
  GPX92_RS10155 aroK 1255149..1255667 (+) 519 WP_078926716.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37911.13 Da        Isoelectric Point: 6.9695

>NTDB_id=408057 GPX92_RS10130 WP_078926721.1 1250520..1251542(+) (pilM) [Vibrio cincinnatiensis strain 2070-81]
MGKSLVTGVDIGHHSIKAVVLKLSGDTYSLVSYKEIAVTGDIFADNYTLDYQKIVKKLKELKKSLPLFNRKVSIAVPDNS
VISKILQIESDLEPAEQEFAIYQAFSHQSPLPIDDLSLDFIKISDNVLGRSRSATYQVYATRREVVESRISASEKAGLNP
VLLDVQMHSLVRIWQQVSQIQQRTNWMLLDIGYSQSSLCLDFIDKAPFCKEIPLGTRWFNQTEQSEGSHDVASLDSSTDV
QAFNQEFVERLARQIQLFTSVHGTHSLAGIWLSGGGASISGLAEAISERLALPCERFTPLSIFSHKLNKRRRTLNEQQRF
STAAGLALRGIEWLESGHAA

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=408057 GPX92_RS10130 WP_078926721.1 1250520..1251542(+) (pilM) [Vibrio cincinnatiensis strain 2070-81]
ATGGGTAAATCATTAGTTACAGGTGTTGATATTGGGCATCACAGCATTAAAGCTGTGGTTTTAAAGTTAAGTGGCGATAC
CTATTCGCTCGTTAGCTATAAAGAAATTGCCGTAACCGGCGACATTTTCGCCGATAACTACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTCAAAAAGAGCCTACCTTTGTTTAACCGCAAGGTCTCTATTGCCGTACCTGATAACTCG
GTCATCAGCAAAATATTGCAAATAGAAAGTGATCTTGAACCCGCTGAGCAAGAATTTGCTATTTATCAGGCGTTCTCTCA
TCAGTCACCATTACCGATTGACGATCTGAGTTTAGATTTCATTAAGATATCAGATAACGTACTGGGACGAAGTCGTAGTG
CAACGTATCAAGTCTATGCTACGCGCCGTGAAGTGGTGGAAAGCCGTATTTCTGCTTCTGAAAAGGCAGGCCTTAATCCA
GTACTTCTTGATGTACAGATGCACAGTTTAGTGCGTATCTGGCAGCAAGTGAGCCAAATTCAGCAGAGAACGAATTGGAT
GTTACTTGATATTGGCTATAGCCAAAGTTCTTTGTGCCTTGATTTCATCGATAAAGCCCCATTTTGTAAAGAGATACCAC
TGGGTACTCGTTGGTTTAACCAAACTGAGCAGTCAGAGGGAAGTCATGATGTTGCATCGTTGGACAGCTCAACCGATGTA
CAAGCCTTTAATCAAGAGTTTGTTGAACGATTGGCGCGGCAAATTCAGCTATTTACTTCCGTACATGGCACACACAGTTT
GGCGGGAATATGGCTTTCTGGAGGGGGAGCTTCTATCTCCGGATTAGCGGAGGCGATTAGTGAGCGACTCGCATTACCAT
GTGAGCGGTTTACACCGCTCTCTATCTTTAGTCATAAATTGAATAAGCGTCGGCGAACGTTAAATGAGCAGCAACGATTC
AGTACCGCTGCTGGGTTAGCGTTACGAGGTATTGAATGGTTGGAGAGTGGGCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QY68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

62.647

100

0.626
  pilM Vibrio campbellii strain DS40M4

48.824

100

0.488

Multiple sequence alignment