Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY29_RS16805 Genome accession   NZ_CP046787
Coordinates   3351584..3352600 (-) Length   338 a.a.
NCBI ID   WP_029837357.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1174     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3346584..3357600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY29_RS16780 aroK 3347444..3347962 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY29_RS16785 pilQ 3348151..3349899 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY29_RS16790 pilP 3349931..3350446 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  GPY29_RS16795 pilO 3350436..3351029 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY29_RS16800 pilN 3351022..3351600 (-) 579 WP_029837358.1 PilN domain-containing protein Machinery gene
  GPY29_RS16805 pilM 3351584..3352600 (-) 1017 WP_029837357.1 type IV pilus assembly protein PilM Machinery gene
  GPY29_RS16810 - 3352743..3355313 (+) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  GPY29_RS16815 oxyR 3355369..3356277 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GPY29_RS16820 - 3356448..3357176 (+) 729 WP_029837356.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37861.42 Da        Isoelectric Point: 7.0224

>NTDB_id=407800 GPY29_RS16805 WP_029837357.1 3351584..3352600(-) (pilM) [Vibrio parahaemolyticus strain 2013V-1174]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMRTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407800 GPY29_RS16805 WP_029837357.1 3351584..3352600(-) (pilM) [Vibrio parahaemolyticus strain 2013V-1174]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGAGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment