Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY39_RS12510 Genome accession   NZ_CP046783
Coordinates   2467403..2468419 (-) Length   338 a.a.
NCBI ID   WP_021449761.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1181     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2462403..2473419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY39_RS12485 aroK 2463263..2463781 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY39_RS12490 pilQ 2463970..2465718 (-) 1749 WP_025442303.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY39_RS12495 pilP 2465750..2466265 (-) 516 WP_021449759.1 pilus assembly protein PilP Machinery gene
  GPY39_RS12500 pilO 2466255..2466848 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY39_RS12505 pilN 2466841..2467419 (-) 579 WP_021449760.1 PilN domain-containing protein Machinery gene
  GPY39_RS12510 pilM 2467403..2468419 (-) 1017 WP_021449761.1 type IV pilus assembly protein PilM Machinery gene
  GPY39_RS12515 - 2468562..2471132 (+) 2571 WP_025622219.1 PBP1A family penicillin-binding protein -
  GPY39_RS12520 oxyR 2471188..2472096 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GPY39_RS12525 - 2472267..2472995 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=407701 GPY39_RS12510 WP_021449761.1 2467403..2468419(-) (pilM) [Vibrio parahaemolyticus strain 2013V-1181]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407701 GPY39_RS12510 WP_021449761.1 2467403..2468419(-) (pilM) [Vibrio parahaemolyticus strain 2013V-1181]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment