Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   MS8324_RS10735 Genome accession   NZ_CP058917
Coordinates   2143146..2144573 (+) Length   475 a.a.
NCBI ID   WP_001538915.1    Uniprot ID   -
Organism   Escherichia coli strain MS8324     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2138146..2149573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS8324_RS10705 (MS8324_2084) pduU 2138525..2138875 (+) 351 WP_000211397.1 propanediol utilization microcompartment protein PduU -
  MS8324_RS10710 (MS8324_2085) - 2138880..2139323 (+) 444 WP_001189443.1 EutP/PduV family microcompartment system protein -
  MS8324_RS10715 (MS8324_2086) tmaR 2139423..2139752 (-) 330 WP_000450409.1 DUF496 family protein -
  MS8324_RS10720 (MS8324_2087) yeeA 2139923..2140981 (-) 1059 WP_001200890.1 FUSC family protein -
  MS8324_RS10725 (MS8324_2088) sbmC 2141179..2141652 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  MS8324_RS10730 (MS8324_2089) dacD 2141771..2142937 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  MS8324_RS10735 (MS8324_2090) sbcB 2143146..2144573 (+) 1428 WP_001538915.1 exodeoxyribonuclease I Machinery gene
  MS8324_RS10740 (MS8324_2091) plaP 2144647..2146005 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  MS8324_RS10745 yoeI 2145995..2146057 (-) 63 WP_010723108.1 membrane protein YoeI -
  MS8324_RS10750 (MS8324_2092) yeeY 2146272..2147201 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  MS8324_RS10755 (MS8324_2093) yeeZ 2147247..2148071 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  MS8324_RS10760 (MS8324_2094) yoeB 2148154..2148408 (-) 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  MS8324_RS10765 (MS8324_2095) yefM 2148405..2148656 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  MS8324_RS10770 hisL 2148939..2148989 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54483.79 Da        Isoelectric Point: 5.4720

>NTDB_id=407623 MS8324_RS10735 WP_001538915.1 2143146..2144573(+) (sbcB) [Escherichia coli strain MS8324]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=407623 MS8324_RS10735 WP_001538915.1 2143146..2144573(+) (sbcB) [Escherichia coli strain MS8324]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCACCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAGAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment