Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY46_RS23605 Genome accession   NZ_CP046776
Coordinates   2989284..2990300 (-) Length   338 a.a.
NCBI ID   WP_021449761.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain AM43962     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2984284..2995300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY46_RS23580 aroK 2985144..2985662 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY46_RS23585 pilQ 2985851..2987599 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY46_RS23590 pilP 2987631..2988146 (-) 516 WP_025503625.1 pilus assembly protein PilP Machinery gene
  GPY46_RS23595 pilO 2988136..2988729 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY46_RS23600 pilN 2988722..2989300 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  GPY46_RS23605 pilM 2989284..2990300 (-) 1017 WP_021449761.1 type IV pilus assembly protein PilM Machinery gene
  GPY46_RS23610 - 2990443..2993013 (+) 2571 WP_025503624.1 PBP1A family penicillin-binding protein -
  GPY46_RS23615 oxyR 2993069..2993977 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GPY46_RS23620 - 2994148..2994876 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=407532 GPY46_RS23605 WP_021449761.1 2989284..2990300(-) (pilM) [Vibrio parahaemolyticus strain AM43962]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407532 GPY46_RS23605 WP_021449761.1 2989284..2990300(-) (pilM) [Vibrio parahaemolyticus strain AM43962]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment