Detailed information NTG0407351

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HZT32_RS01310
• Genome accession: NZ_CP058874
• Coordinates: 270360..271343 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain STLEFF_47
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 265360..276343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZT32_RS01290 (HZT32_01275)	dppA	266503..268110 (+)	1608	WP_001222876.1	dipeptide ABC transporter substrate-binding protein DppA	-
HZT32_RS01295 (HZT32_01280)	-	268173..268400 (-)	228	WP_001696297.1	hypothetical protein	-
HZT32_RS01300 (HZT32_01285)	dppB	268418..269437 (+)	1020	WP_000938853.1	dipeptide ABC transporter permease DppB	-
HZT32_RS01305 (HZT32_01290)	dppC	269447..270349 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HZT32_RS01310 (HZT32_01295)	amiE	270360..271343 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
HZT32_RS01315 (HZT32_01300)	dppF	271340..272344 (+)	1005	WP_240724426.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HZT32_RS01320 (HZT32_01305)	yhjV	272374..273645 (-)	1272	WP_001318103.1	aromatic amino acid transport family protein	-
HZT32_RS01325 (HZT32_01310)	-	274121..274228 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HZT32_RS01330 (HZT32_01315)	-	274604..274711 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=407351 HZT32_RS01310 WP_001196486.1 270360..271343(+) (amiE) [Escherichia coli strain STLEFF_47]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=407351 HZT32_RS01310 WP_001196486.1 270360..271343(+) (amiE) [Escherichia coli strain STLEFF_47]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGGGAGTCTGGCTCCGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTTGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGATAAAGAACGTCT
GGCGTCGTTGCCAGGTGTTGTTCCCGGCAAGTATGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTTGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404