Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY00_RS07645 Genome accession   NZ_CP046747
Coordinates   1637873..1638463 (+) Length   196 a.a.
NCBI ID   WP_000157688.1    Uniprot ID   A0A655VUM8
Organism   Vibrio cholerae strain 3541-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632873..1643463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY00_RS07630 - 1633670..1636147 (-) 2478 WP_199252369.1 penicillin-binding protein 1A -
  GPY00_RS07635 pilM 1636290..1637306 (+) 1017 WP_158128539.1 type IV pilus assembly protein PilM Machinery gene
  GPY00_RS07640 pilN 1637296..1637880 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  GPY00_RS07645 pilO 1637873..1638463 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY00_RS07650 pilP 1638453..1638971 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  GPY00_RS07655 pilQ 1638997..1640712 (+) 1716 WP_187386856.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY00_RS07660 aroK 1640899..1641423 (+) 525 WP_000818616.1 shikimate kinase AroK -
  GPY00_RS07665 aroB 1641451..1642536 (+) 1086 WP_044127029.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22891.30 Da        Isoelectric Point: 4.4169

>NTDB_id=407061 GPY00_RS07645 WP_000157688.1 1637873..1638463(+) (pilO) [Vibrio cholerae strain 3541-04]
MASLQELELDEISEWPLLPQLLVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=407061 GPY00_RS07645 WP_000157688.1 1637873..1638463(+) (pilO) [Vibrio cholerae strain 3541-04]
ATGGCTAGTTTGCAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGCTGCTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATTCAAGCTCAGTTGGATGAG
CTCAAAGAGCGTTACGATTTTTTACTCCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGAGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

99.49

100

0.995
  pilO Vibrio campbellii strain DS40M4

71.429

96.429

0.689

Multiple sequence alignment