Detailed information NTG0406893

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HZS87_RS18920
• Genome accession: NZ_CP058725
• Coordinates: 3874105..3875085 (+)
• Length: 326 a.a.
• NCBI ID: WP_001618842.1
• Uniprot ID: -
• Organism: Escherichia coli strain S171-1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3869105..3880085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZS87_RS18890	yggM	3869620..3870627 (+)	1008	WP_001460056.1	DUF1202 family protein	-
HZS87_RS18895	hemW	3870782..3871918 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
HZS87_RS18900	rdgB	3871911..3872504 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
HZS87_RS18905	yggU	3872512..3872802 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
HZS87_RS18910	yggT	3872799..3873365 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
HZS87_RS18915	yggS	3873383..3874087 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
HZS87_RS18920	pilT	3874105..3875085 (+)	981	WP_001618842.1	type IV pilus twitching motility protein PilT	Machinery gene
HZS87_RS18925	ruvX	3875269..3875685 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
HZS87_RS18930	yqgE	3875685..3876248 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
HZS87_RS18935	gshB	3876357..3877307 (-)	951	WP_000593273.1	glutathione synthase	-
HZS87_RS18940	rsmE	3877320..3878051 (-)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
HZS87_RS18945	endA	3878131..3878838 (-)	708	WP_000286500.1	deoxyribonuclease I	-
HZS87_RS18950	yggI	3878933..3879430 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35937.10 Da
• Isoelectric Point: 5.9172

>NTDB_id=406893 HZS87_RS18920 WP_001618842.1 3874105..3875085(+) (pilT) [Escherichia coli strain S171-1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=406893 HZS87_RS18920 WP_001618842.1 3874105..3875085(+) (pilT) [Escherichia coli strain S171-1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.453	97.546	0.414
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.747	100	0.374
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362