Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   GPW66_RS05260 Genome accession   NZ_CP046736
Coordinates   127913..128920 (-) Length   335 a.a.
NCBI ID   WP_000116735.1    Uniprot ID   A0A085SEX1
Organism   Vibrio cholerae strain 3528-08     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 122913..133920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS05250 cysM 124076..124963 (+) 888 WP_001888660.1 cysteine synthase CysM -
  GPW66_RS05255 mutS 125231..127819 (+) 2589 WP_158106282.1 DNA mismatch repair protein MutS -
  GPW66_RS05260 rpoS 127913..128920 (-) 1008 WP_000116735.1 RNA polymerase sigma factor RpoS Regulator
  GPW66_RS05265 nlpD 128994..129929 (-) 936 WP_000177561.1 murein hydrolase activator NlpD -
  GPW66_RS05270 - 129929..130555 (-) 627 WP_000002982.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  GPW66_RS05275 surE 130548..131300 (-) 753 WP_032472724.1 5'/3'-nucleotidase SurE -
  GPW66_RS05280 truD 131302..132387 (-) 1086 WP_001888656.1 tRNA pseudouridine(13) synthase TruD -
  GPW66_RS05285 ispF 132417..132893 (-) 477 WP_032472723.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GPW66_RS05290 ispD 132890..133588 (-) 699 WP_001888653.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GPW66_RS05295 ftsB 133585..133869 (-) 285 WP_001263189.1 cell division protein FtsB -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 38504.37 Da        Isoelectric Point: 4.3648

>NTDB_id=406813 GPW66_RS05260 WP_000116735.1 127913..128920(-) (rpoS) [Vibrio cholerae strain 3528-08]
MSVSNTVTKVEEFDFEDEALEVLETDAELASDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFNVEYDN

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=406813 GPW66_RS05260 WP_000116735.1 127913..128920(-) (rpoS) [Vibrio cholerae strain 3528-08]
ATGAGTGTCAGCAATACCGTAACCAAAGTAGAAGAGTTCGATTTTGAAGATGAAGCACTGGAAGTGCTAGAAACTGATGC
CGAGCTCGCCAGTGATGAAGAATTAGTTGCTGTTGAAGGGGCAAGTGAAGACGTTCGTGAAGAGTTTGATGCTTCTGCGA
AAAGTCTTGATGCGACCCAGATGTATCTCAGCGAAATTGGTTTTTCACCGCTCCTTACTGCCGAAGAAGAAGTGCTTTAT
GCTCGTCGTGCCTTACGTGGTGATGAAGCCGCACGTAAACGCATGATTGAAAGTAACTTGCGTCTGGTGGTAAAAATTTC
ACGCCGTTACAGCAACCGAGGATTAGCACTGCTCGATCTGATTGAAGAAGGTAATCTTGGTCTGATCCGTGCGGTTGAGA
AATTCGATCCAGAACGCGGTTTCCGCTTCTCTACCTACGCAACATGGTGGATCCGTCAAACCATTGAACGTGCGCTAATG
AACCAAACACGCACCATTCGTCTACCGATTCATGTCGTCAAAGAGCTGAACATTTATTTGCGTACTGCTCGCGAATTATC
ACAGCGCCTTGACCACGAACCTACGCCAGAAGAAATTGCTCTTGAGTTAGATCGACCTGTCGATGATGTGACTAAGATGC
TGCGTCTTAACGAACGGATCAGCTCAGTGGATACGCCAATTGGTGGGGATGGAGATAAGGCACTGCTGGATATTTTGCCA
GACTCTCACAATGCCGATCCTGAGTTTTCAACTCAAGATGATGACATTCGTGAATCGCTGCTCAACTGGTTGGATGAACT
TAATCCAAAGCAAAAAGAAGTGCTTGCGCGTCGCTTTGGGCTTCTTGGCTATGAACCATCGACCTTGGAAGAAGTGGGTC
GTGAGATCAATCTCACTCGTGAGCGTGTTCGCCAAATCCAAGTGGAAGGTCTACGTCGTCTGCGTGAGATTTTGGTGAAA
CAAGGTTTGAATATGGAAGCGCTGTTTAACGTCGAATACGACAACTAA

Domains

Predicted by InterproScan.

Sigma-70 region 3 (Sigma70_r3)

(175-249)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(57-90)

Sigma-70 region 2 (Sigma70_r2)

(95-164)

Sigma-70, region 4 (Sigma70_r4)

(263-316)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A085SEX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

99.701

100

0.997

Multiple sequence alignment