Detailed information NTG0406808

Bioinformatically predicted

Overview

• Name: chiS
• Type: Regulator
• Locus tag: GPW66_RS04830
• Genome accession: NZ_CP046736
• Coordinates: 32042..35575 (+)
• Length: 1177 a.a.
• NCBI ID: WP_001888565.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain 3528-08
• Function: chitin sensor (predicted from homology) Competence regulation

Genomic Context

Location: 27042..40575

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPW66_RS04805	erpA	27621..27962 (-)	342	WP_000005741.1	iron-sulfur cluster insertion protein ErpA	-
GPW66_RS04810	hemL	28246..29544 (+)	1299	WP_000167257.1	glutamate-1-semialdehyde 2,1-aminomutase	-
GPW66_RS04820	-	29798..30883 (+)	1086	WP_001290812.1	AI-2E family transporter	-
GPW66_RS04825	rsmC	30956..31978 (+)	1023	WP_001888567.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
GPW66_RS04830	chiS	32042..35575 (+)	3534	WP_001888565.1	two-component system sensor histidine kinase ChiS	Regulator
GPW66_RS04835	-	36486..38156 (+)	1671	WP_001888564.1	ABC transporter substrate-binding protein	-
GPW66_RS04840	-	38326..39312 (+)	987	WP_001888563.1	ABC transporter permease	-
GPW66_RS04845	-	39315..40340 (+)	1026	WP_001888562.1	ABC transporter permease	-

Sequence

Protein

• Length: 1177 a.a.
• Molecular weight: 133138.29 Da
• Isoelectric Point: 6.8941

>NTDB_id=406808 GPW66_RS04830 WP_001888565.1 32042..35575(+) (chiS) [Vibrio cholerae strain 3528-08]
MRLCPRFSLDLLVKVKKLVNFVTLGFSCLSPTHAWRSPQSFTESNTLIMFRFYRKQKFKRLQNTLMAAFLALSIIPLTIT
ALFFLHSHSKDLEQQSTSYLVSVRDNKQQQVIDYMMAKESEVMGFVRSELAYASGGRFYGLVNAFQRLDVSIEAAREHAQ
QRYITGSGDQIKTSVLPQSSSYVGSERYRLLHKRYHWAYLELLKRSDFDDILLVDIDGNVVYSIYKYDNFGTNLLTGKYQ
DTNLGHTFKRLEQTVNEQRKTNEDFTPVVISDFVQEDGKQYAWLGAPIIQQGYLHSYAMFRLPSNAITKLIAEGSSNPSM
QTILVGQDHRSRTLTSAEIAVEKSKAVVDLALSGKRAVGTYTNTDGEQIIAAYAPINLKNIHWALVVELSEKEAFARVRQ
LEKLFVFAMLTAIVLVVIASHYLSNFITSPLLKLTWAAERVSAGDLDEAMINTERKDEIGRLAVSFERMQRSIREKISLI
KSQNKELESNLLIIRKQNDELQLANKLKDEFLATTSHELRTPLHGMIGIAEALISGANGPISAAHKYQLDIIISSGQRLA
TLVDDLLDYHKMRYGALDIQRCAVDLSSATRLVLELSHHLLGKKTLRIINQVSEQPVWVSADPQRLEQVLYNLIGNAIKY
TSEGKIVISATYIDDKVRVQVVDTGQGIPAEQLEHIFEPLIQAGHDASRYRQGAGLGLSISRQLIELMHGTLYVSSQPMV
GTTFSFTLPLASEEEIAQARLTELPHFQAPEVLDTELPEQSNLPENEHGPLLLVADDEPVNLRVLDSFLRLEGYRVHTAQ
DGHQVLEAIKREKPELVLLDIMMPGMSGYQVCEKLRQTYDHAELPIIMLTALNQSDDRVRGFEAGANDYLSKPFNKQELA
ARIVAHLTASKAELRRIENAQLQKELKHRAMVEASLLETQGRLLEQLESAPEAILCVKEGNKVRFANEAAARLFRRTPEQ
LKRSNAEELIAPKFLNIEQEHYCGNIDVYVDDVRQHLSADVLRLPQGSGLQAMYIFNVGGSVNAARIHNLETAVEALSSY
AFEGDKDKLQKLKELGGEFTRLADKASGEYQSKQDLMRAVLVEAMTSALNYWERVSGQSKFTFAEQSGLWRVYLDRSTLQ
TRTLDKYLRIETLPKTPRWRTVLNSLDYILEHCKEAGPERTHIEMQRDKLQKLLTSE

Nucleotide

• Length: 3534 bp

>NTDB_id=406808 GPW66_RS04830 WP_001888565.1 32042..35575(+) (chiS) [Vibrio cholerae strain 3528-08]
TTGCGTCTATGCCCACGCTTTAGCTTGGACTTACTTGTCAAAGTAAAAAAACTCGTTAATTTCGTTACTTTAGGTTTTTC
TTGTCTATCACCCACTCATGCTTGGCGATCGCCGCAGAGTTTCACCGAAAGTAACACCTTAATTATGTTTAGGTTCTATC
GAAAACAAAAATTCAAACGCCTGCAAAATACCTTGATGGCTGCGTTTTTGGCCCTCAGCATTATTCCTTTGACCATTACT
GCGTTGTTTTTCCTACATTCACACAGTAAAGATCTGGAGCAGCAGAGCACCTCTTACCTCGTTTCTGTTCGGGATAATAA
ACAGCAACAGGTCATCGACTACATGATGGCCAAAGAGTCGGAAGTGATGGGTTTTGTTCGCTCCGAGCTGGCTTATGCCA
GTGGCGGGCGTTTTTATGGTCTGGTGAATGCTTTCCAGCGTTTAGACGTCAGTATTGAAGCGGCTCGTGAACATGCTCAG
CAGCGCTATATCACCGGATCGGGTGACCAAATAAAAACCTCGGTGCTCCCCCAATCAAGCAGCTACGTGGGCAGTGAACG
CTATCGACTGCTACATAAACGCTACCACTGGGCTTACTTAGAACTACTGAAACGGTCGGACTTTGATGACATTTTATTGG
TCGATATTGATGGCAACGTCGTTTATTCGATTTATAAATACGACAATTTTGGCACCAACTTATTAACGGGCAAATACCAA
GACACCAATCTAGGCCATACCTTTAAGCGTTTAGAACAAACGGTCAATGAACAGCGCAAAACCAACGAAGATTTCACCCC
TGTCGTGATTTCTGATTTCGTACAAGAGGATGGTAAACAATACGCTTGGCTTGGCGCACCGATTATTCAGCAAGGCTACT
TGCATAGTTACGCGATGTTCCGCTTACCAAGCAATGCGATCACCAAACTCATCGCAGAAGGCAGTAGCAATCCTTCGATG
CAAACCATTCTGGTGGGACAAGATCACCGCTCAAGAACCTTAACTTCTGCCGAAATTGCGGTTGAAAAAAGTAAAGCTGT
GGTCGATTTAGCCTTATCGGGCAAACGCGCCGTAGGCACTTACACCAATACCGATGGTGAACAGATCATCGCGGCCTACG
CACCGATCAATTTGAAAAATATCCATTGGGCTCTGGTGGTTGAACTGTCAGAGAAAGAAGCGTTTGCTCGCGTTCGTCAG
TTAGAAAAACTGTTTGTTTTTGCGATGTTGACGGCTATCGTCTTAGTGGTCATCGCCTCGCATTACCTTTCCAATTTCAT
TACCTCACCGCTTTTGAAGCTGACATGGGCAGCCGAACGTGTTTCGGCAGGCGATCTTGATGAAGCCATGATCAATACCG
AACGCAAAGATGAAATTGGCCGCTTGGCGGTCAGTTTTGAGCGTATGCAGCGTTCCATTCGCGAAAAAATATCGCTGATC
AAATCGCAAAATAAAGAGTTAGAGAGCAACTTACTGATCATTCGTAAGCAAAACGATGAGTTACAGCTAGCGAATAAGCT
AAAAGATGAGTTCCTCGCGACCACATCACATGAATTACGCACCCCATTGCATGGCATGATTGGTATTGCCGAAGCGCTGA
TCTCGGGGGCAAATGGCCCGATTTCAGCAGCGCACAAGTATCAACTCGACATCATTATCAGCAGTGGGCAGCGCTTGGCG
ACTCTGGTTGATGATCTACTCGATTACCACAAAATGCGCTATGGCGCGCTGGATATTCAAAGGTGTGCCGTCGATTTATC
GAGCGCTACGCGCTTAGTCTTGGAGCTTTCTCACCATTTACTTGGCAAGAAAACGCTGCGCATTATTAATCAAGTCTCAG
AACAACCGGTCTGGGTTTCTGCGGATCCTCAACGCTTAGAGCAAGTCCTGTATAACTTGATCGGCAATGCCATTAAGTAC
ACTTCCGAAGGCAAAATTGTTATCTCCGCAACCTATATTGACGATAAAGTGCGCGTGCAGGTGGTGGATACAGGTCAAGG
GATTCCGGCAGAACAACTGGAGCACATCTTTGAGCCATTGATTCAAGCGGGCCATGATGCGAGTCGCTATCGCCAAGGCG
CAGGACTCGGGCTTTCCATCAGTCGTCAGTTAATCGAATTGATGCACGGCACTCTCTACGTAAGTAGTCAGCCGATGGTT
GGCACTACCTTTAGCTTCACGCTGCCTCTGGCGAGCGAAGAAGAGATTGCTCAAGCGCGCTTGACCGAGCTCCCCCACTT
CCAAGCACCGGAAGTGTTGGATACTGAACTGCCCGAGCAAAGCAACTTACCCGAAAATGAACATGGCCCACTTCTGTTGG
TCGCCGATGATGAACCAGTCAACCTGCGTGTGCTGGACAGTTTTTTACGTCTTGAAGGTTATCGCGTACATACCGCGCAA
GATGGCCATCAAGTACTTGAAGCAATTAAACGTGAAAAACCTGAATTGGTGTTACTCGACATCATGATGCCAGGCATGAG
CGGTTATCAAGTGTGTGAAAAACTTCGACAAACTTACGACCACGCTGAACTGCCTATTATTATGCTGACGGCTCTCAACC
AATCTGATGACCGGGTACGAGGGTTTGAAGCTGGAGCCAATGACTACTTATCCAAACCATTCAACAAACAAGAGCTGGCC
GCGCGCATTGTCGCTCACCTCACGGCGAGCAAAGCCGAACTGCGCCGCATTGAGAATGCCCAACTGCAAAAAGAGTTGAA
ACATCGCGCTATGGTAGAAGCGAGCCTATTGGAAACCCAAGGCCGATTATTGGAACAGTTAGAATCTGCGCCAGAAGCGA
TTTTGTGCGTTAAAGAGGGCAATAAAGTACGTTTTGCTAACGAAGCGGCAGCACGTTTATTTAGACGTACCCCTGAGCAG
CTCAAACGCTCCAACGCCGAAGAACTGATTGCTCCTAAATTCTTGAATATTGAGCAAGAGCATTACTGCGGCAATATTGA
TGTTTACGTAGATGATGTGCGCCAGCACCTCTCTGCCGATGTCCTTCGCTTGCCGCAAGGTTCTGGCTTACAAGCGATGT
ACATTTTTAATGTCGGAGGCAGCGTCAATGCCGCGCGGATTCATAACCTCGAAACCGCGGTAGAAGCTCTGTCTAGCTAT
GCCTTCGAAGGCGATAAAGACAAGCTGCAAAAACTCAAGGAACTGGGTGGTGAGTTTACTCGTCTTGCAGATAAAGCCTC
GGGCGAGTATCAATCGAAACAAGATTTGATGCGTGCCGTGTTAGTCGAAGCCATGACCAGTGCCTTGAACTATTGGGAAC
GAGTCTCAGGGCAAAGCAAGTTCACGTTTGCCGAACAAAGTGGCTTGTGGCGCGTTTATCTTGACCGCAGCACCCTACAA
ACTCGCACCCTAGACAAATACCTACGAATTGAGACACTGCCTAAAACACCGCGTTGGCGAACTGTACTGAACTCGCTCGA
CTATATTCTTGAGCATTGCAAAGAAGCAGGCCCTGAACGCACTCACATCGAAATGCAGCGCGATAAATTGCAAAAACTCC
TGACCAGTGAATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (622-731)

• HAMP domain (HAMP) (423-475)

• Response regulator receiver domain (Response_reg) (773-883)

• His Kinase A (phospho-acceptor) domain (HisKA) (508-573)

• PAS domain (PAS_8) (927-961)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
chiS	Vibrio cholerae strain A1552	99.83	100	0.998