Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GO998_RS15310 Genome accession   NZ_CP046729
Coordinates   3254560..3256059 (+) Length   499 a.a.
NCBI ID   WP_211904037.1    Uniprot ID   -
Organism   Ralstonia syzygii strain LLRS-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3249560..3261059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO998_RS15285 (GO998_15290) - 3250738..3251721 (+) 984 WP_075465395.1 KpsF/GutQ family sugar-phosphate isomerase -
  GO998_RS15290 (GO998_15295) - 3251754..3252341 (+) 588 WP_013213608.1 KdsC family phosphatase -
  GO998_RS15295 (GO998_15300) lptC 3252343..3252975 (+) 633 WP_075465397.1 LPS export ABC transporter periplasmic protein LptC -
  GO998_RS15300 (GO998_15305) lptA 3253044..3253640 (+) 597 WP_134927697.1 lipopolysaccharide transport periplasmic protein LptA -
  GO998_RS15305 (GO998_15310) lptB 3253674..3254462 (+) 789 WP_013213611.1 LPS export ABC transporter ATP-binding protein -
  GO998_RS15310 (GO998_15315) rpoN/rpoN1 3254560..3256059 (+) 1500 WP_211904037.1 RNA polymerase factor sigma-54 Machinery gene
  GO998_RS15315 (GO998_15320) hpf 3256111..3256464 (+) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GO998_RS15320 (GO998_15325) ptsN 3256812..3257267 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GO998_RS15325 (GO998_15330) hprK 3257338..3258312 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  GO998_RS15330 (GO998_15335) - 3258502..3258831 (+) 330 WP_075465401.1 PsiF family protein -
  GO998_RS15335 (GO998_15340) rapZ 3258924..3259814 (+) 891 WP_013213615.1 RNase adapter RapZ -
  GO998_RS15340 (GO998_15345) - 3259894..3260544 (+) 651 WP_134927698.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55093.49 Da        Isoelectric Point: 4.8496

>NTDB_id=406783 GO998_RS15310 WP_211904037.1 3254560..3256059(+) (rpoN/rpoN1) [Ralstonia syzygii strain LLRS-1]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPAANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPVELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGSANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=406783 GO998_RS15310 WP_211904037.1 3254560..3256059(+) (rpoN/rpoN1) [Ralstonia syzygii strain LLRS-1]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGTCGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGCCTGAAGAAAGCGTTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGTGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCTCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.194

100

0.972

Multiple sequence alignment