Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GO998_RS09660 Genome accession   NZ_CP046729
Coordinates   2073680..2075044 (-) Length   454 a.a.
NCBI ID   WP_118883301.1    Uniprot ID   -
Organism   Ralstonia syzygii strain LLRS-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2068680..2080044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO998_RS09645 (GO998_09640) - 2069745..2071691 (+) 1947 WP_211903553.1 ATP-binding cassette domain-containing protein -
  GO998_RS09650 (GO998_09645) - 2071723..2072829 (+) 1107 WP_211903554.1 CaiB/BaiF CoA transferase family protein -
  GO998_RS09655 (GO998_09655) - 2073107..2073586 (-) 480 WP_075464533.1 disulfide bond formation protein B -
  GO998_RS09660 (GO998_09660) radA 2073680..2075044 (-) 1365 WP_118883301.1 DNA repair protein RadA Machinery gene
  GO998_RS09665 (GO998_09665) alr 2075129..2076256 (-) 1128 WP_211903555.1 alanine racemase -
  GO998_RS09670 (GO998_09670) - 2076255..2076470 (+) 216 WP_211903556.1 hypothetical protein -
  GO998_RS09675 (GO998_09675) lplT 2076885..2078201 (+) 1317 WP_211903557.1 lysophospholipid transporter LplT -
  GO998_RS09680 (GO998_09680) - 2078271..2079092 (-) 822 WP_211903558.1 uracil-DNA glycosylase -
  GO998_RS09685 (GO998_09685) rimI 2079163..2079690 (-) 528 WP_211903559.1 ribosomal protein S18-alanine N-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48302.74 Da        Isoelectric Point: 7.6668

>NTDB_id=406773 GO998_RS09660 WP_118883301.1 2073680..2075044(-) (radA) [Ralstonia syzygii strain LLRS-1]
MAKSKTVYTCTECGGTSPKWAGQCPHCQQWNTLVETVAQPAAGARFQSLAANATVRKLADIDAVDVPRFSSGIDEFDRVL
GGGLVGGGVVLIGGDPGIGKSTLLLQALSNLAASRRVLYVSGEESGAQIALRARRLGIESPNLALLAEIQLERIQATIEA
EKPEVVVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRVAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGDTH
SAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQTVPGSCVLVTQEGTRPLLVEIQALVDAAGVPNPRR
LAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPRGLVVFGEIGLAGEIRP
TPRGQERLKEAAKLGFSMAVIPKSNAPKQPIDGLQVIAVERIEQAIDRVRGLET

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=406773 GO998_RS09660 WP_118883301.1 2073680..2075044(-) (radA) [Ralstonia syzygii strain LLRS-1]
TTGGCCAAGAGCAAGACGGTCTATACGTGCACCGAATGCGGTGGCACGTCACCGAAATGGGCGGGGCAGTGCCCGCATTG
CCAGCAGTGGAACACGCTGGTGGAAACCGTGGCGCAGCCGGCCGCCGGCGCGCGTTTCCAGTCGCTGGCGGCGAATGCCA
CGGTGCGCAAGCTGGCGGACATCGACGCGGTGGACGTGCCGCGCTTCTCCAGCGGCATCGATGAATTCGATCGCGTGCTG
GGCGGCGGCCTGGTCGGCGGCGGCGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGGC
GCTGTCCAACCTGGCGGCCAGCCGGCGCGTGCTGTACGTGAGCGGCGAGGAGTCCGGCGCGCAGATCGCGCTGCGGGCGC
GGCGGCTGGGTATCGAAAGCCCGAACCTGGCGCTGCTGGCCGAGATCCAGCTCGAGCGCATCCAGGCGACCATCGAGGCG
GAGAAGCCCGAGGTGGTCGTCATCGATTCGATCCAGACGCTGTACTCGGAGGCGCTGACCTCGGCGCCGGGCTCGGTGGC
CCAGGTGCGCGAATGCGCGGCGCAGCTCACGCGCGTTGCCAAGCGGCTGGACGTGACCACCATCCTGGTCGGCCATGTGA
CCAAGGAAGGTGCGCTGGCGGGCCCGCGCGTGCTGGAGCACATCGTCGACACGGTGCTGTATTTCGAGGGCGATACGCAT
TCGGCCTACCGGCTGGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGTGTGTTCGCCATGACCGAGCG
CGGCCTGCGCGGCGTGGCGAATCCGTCGGCGCTGTTCCTGTCGCAGCACGAGCAGACCGTGCCGGGCTCGTGCGTGCTGG
TGACGCAGGAGGGCACGCGCCCGCTGCTGGTGGAGATCCAGGCGCTGGTGGATGCGGCGGGCGTGCCGAACCCGCGGCGG
CTGGCGGTGGGCCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGCGGGCATCGCCTGTTTCGA
CCAGGATGTGTTCCTCAATGCCGTGGGCGGGGTCAAGATCACCGAACCGGCGGCGGACCTGGCGGTGCTGCTGGCGATCC
ATTCGTCGATGCGCAACAAGCCGCTGCCGCGCGGGCTGGTGGTGTTCGGCGAGATCGGGCTGGCCGGCGAGATCCGCCCG
ACGCCGCGCGGGCAGGAGCGCCTGAAGGAAGCGGCCAAGCTGGGCTTCTCGATGGCCGTGATCCCCAAGTCGAATGCGCC
CAAGCAGCCGATCGACGGCCTGCAGGTGATTGCCGTCGAGCGTATCGAGCAGGCGATCGACCGGGTGCGCGGGCTGGAAA
CTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(62-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(360-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.493
  radA Streptococcus pneumoniae Rx1

47.033

100

0.471
  radA Streptococcus pneumoniae D39

47.033

100

0.471
  radA Streptococcus pneumoniae R6

47.033

100

0.471
  radA Streptococcus pneumoniae TIGR4

47.033

100

0.471
  radA Streptococcus mitis SK321

47.228

99.339

0.469
  radA Streptococcus mitis NCTC 12261

47.007

99.339

0.467

Multiple sequence alignment