Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PAASB05_RS16135 Genome accession   NZ_CP046722
Coordinates   3457728..3459110 (-) Length   460 a.a.
NCBI ID   WP_031593287.1    Uniprot ID   -
Organism   Pantoea agglomerans strain ASB05     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3452728..3464110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAASB05_RS16110 (PAASB05_16120) - 3453502..3453666 (+) 165 WP_109652447.1 YhfG family protein -
  PAASB05_RS16115 (PAASB05_16125) - 3453679..3454281 (+) 603 WP_061703613.1 putative adenosine monophosphate-protein transferase Fic -
  PAASB05_RS16120 (PAASB05_16130) - 3454262..3455158 (-) 897 WP_010253616.1 LysR family transcriptional regulator -
  PAASB05_RS16125 (PAASB05_16135) - 3455364..3456365 (+) 1002 WP_158132015.1 zinc-binding alcohol dehydrogenase family protein -
  PAASB05_RS16130 (PAASB05_16140) nadR 3456411..3457652 (-) 1242 WP_010253612.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  PAASB05_RS16135 (PAASB05_16145) radA 3457728..3459110 (-) 1383 WP_031593287.1 DNA repair protein RadA Machinery gene
  PAASB05_RS16140 (PAASB05_16150) serB 3459124..3460101 (-) 978 WP_033770935.1 phosphoserine phosphatase -
  PAASB05_RS16145 (PAASB05_16155) - 3460209..3460859 (+) 651 WP_010253606.1 YtjB family periplasmic protein -
  PAASB05_RS16150 (PAASB05_16160) yjjG 3461526..3462206 (-) 681 WP_010253605.1 pyrimidine 5'-nucleotidase -
  PAASB05_RS16155 (PAASB05_16165) rimI 3462225..3462665 (-) 441 WP_010253603.1 ribosomal protein S18-alanine N-acetyltransferase -
  PAASB05_RS16160 (PAASB05_16170) - 3462637..3463047 (-) 411 WP_022625367.1 DNA polymerase III subunit psi -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49307.93 Da        Isoelectric Point: 7.4220

>NTDB_id=406730 PAASB05_RS16135 WP_031593287.1 3457728..3459110(-) (radA) [Pantoea agglomerans strain ASB05]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGSPGAGKSTLLLQVMCRLSEGMKTLYVTGEESLQQVAMRAHRLGLPTENVNMLSETSIE
QICLIAEQEQPQLMVIDSIQVMHMAEIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEVTSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERIAEAAKHGFKRAIVPAGNAPKKPIAGMQVFSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=406730 PAASB05_RS16135 WP_031593287.1 3457728..3459110(-) (radA) [Pantoea agglomerans strain ASB05]
TTGGCCAAAGCGGCAAAACGCGCATTTGTCTGTAACGAATGTGGCGCAGATTACCCACGCTGGCAGGGGCAGTGCAGCGC
CTGTCACGCCTGGAACACCATCACCGAGGTGCGCATTGCCGCCTCGCCCGCGGCTGCGCGTAACGAGCGCCTGAGTGGCT
ATGCTGGCAGCGCCGGCACCAGCCGGGTGCAGAAGCTCTCTGAAATCAGCCTGGAGGCGTTACCGCGCTTCTCGACCAGC
TTCAAAGAGTTTGACCGGGTGCTGGGCGGCGGGGTGGTGCCGGGCAGCGCCATTCTGATTGGCGGCAGCCCCGGCGCAGG
CAAATCCACGCTGCTGCTGCAGGTGATGTGCCGGTTGTCAGAGGGGATGAAAACCCTGTATGTCACCGGTGAAGAGTCAC
TGCAGCAGGTGGCGATGCGCGCCCATCGTCTGGGATTACCTACCGAAAATGTGAATATGCTGTCGGAAACCAGCATTGAG
CAGATCTGCCTGATCGCCGAACAGGAGCAGCCGCAACTGATGGTGATCGACTCGATCCAGGTAATGCACATGGCGGAGAT
CCAGTCATCGCCCGGCAGCGTCGCCCAGGTGCGTGAAACCGCCGCTTACCTGACGCGCTTCGCCAAGACGCGCGGCGTGG
CGATCATCATGGTCGGTCACGTCACCAAAGATGGCTCCCTGGCCGGACCGAAAGTACTGGAGCACTGTATCGACTGTTCA
GTGCTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGTCATAAAAACCGCTTCGGCGCGGTCAATGAACT
GGGTGTGTTTGCCATGACCGAGCAGGGCATGCGTGAAGTCAGCAACCCGTCAGCTATTTTCCTGTCGCGCGGCGAAGAGG
TCACCTCGGGCAGTTCGGTGATGGTGCTGTGGGAGGGAACGCGACCGCTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGGCAATCCACGCCGCGTGGCAGTGGGTCTGGAGCAGAACCGCCTGGCGATTCTGCTGGCAGTATTGCACCG
TCACGGCGGATTGCAAATGGCGGATCAGGATGTGTTCGTTAACGTGGTCGGCGGCGTCAAAGTCACTGAAACCAGTGCTG
ACCTGGCGCTGATGCTGTCGATGGTGTCGAGCCTGCGCGATCGTCCGTTGCCGCAGGATCTGGTGATCTTCGGCGAAGTG
GGACTGGCGGGCGAAATCCGTCCGGTGCCCAGCGGTCAGGAGCGCATCGCTGAAGCGGCGAAGCATGGCTTCAAACGCGC
CATTGTGCCGGCAGGGAATGCACCGAAAAAACCGATTGCCGGGATGCAGGTTTTCAGCGCGAAGAAGCTGGCCGATGCGC
TGGCGATTCTGGACGATCTGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)

AAA domain (AAA_25)

(87-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.373

100

0.485
  radA Streptococcus mitis NCTC 12261

44.82

96.522

0.433
  radA Streptococcus pneumoniae Rx1

45.244

93.696

0.424
  radA Streptococcus pneumoniae D39

45.244

93.696

0.424
  radA Streptococcus pneumoniae R6

45.244

93.696

0.424
  radA Streptococcus pneumoniae TIGR4

45.244

93.696

0.424
  radA Streptococcus mitis SK321

45.244

93.696

0.424

Multiple sequence alignment