Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GO999_RS14635 Genome accession   NZ_CP046674
Coordinates   3182528..3184027 (+) Length   499 a.a.
NCBI ID   WP_020831172.1    Uniprot ID   -
Organism   Ralstonia nicotianae strain RS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3177528..3189027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO999_RS14610 (GO999_14610) - 3178721..3179704 (+) 984 WP_016724265.1 SIS domain-containing protein -
  GO999_RS14615 (GO999_14615) - 3179743..3180330 (+) 588 WP_011000373.1 HAD family hydrolase -
  GO999_RS14620 (GO999_14620) lptC 3180332..3180964 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  GO999_RS14625 (GO999_14625) lptA 3181033..3181608 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  GO999_RS14630 (GO999_14630) lptB 3181642..3182430 (+) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  GO999_RS14635 (GO999_14635) rpoN/rpoN1 3182528..3184027 (+) 1500 WP_020831172.1 RNA polymerase factor sigma-54 Machinery gene
  GO999_RS14640 (GO999_14640) hpf 3184079..3184432 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GO999_RS14645 (GO999_14645) ptsN 3184782..3185237 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GO999_RS14650 (GO999_14650) hprK 3185308..3186282 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  GO999_RS14655 (GO999_14655) - 3186473..3186808 (+) 336 WP_020425146.1 PsiF family protein -
  GO999_RS14660 (GO999_14660) rapZ 3186902..3187792 (+) 891 WP_011000365.1 RNase adapter RapZ -
  GO999_RS14665 (GO999_14665) - 3187870..3188520 (+) 651 WP_011000364.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55213.53 Da        Isoelectric Point: 4.8037

>NTDB_id=406662 GO999_RS14635 WP_020831172.1 3182528..3184027(+) (rpoN/rpoN1) [Ralstonia nicotianae strain RS]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTADASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=406662 GO999_RS14635 WP_020831172.1 3182528..3184027(+) (rpoN/rpoN1) [Ralstonia nicotianae strain RS]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACAGCCGACGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment