Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GPK32_RS03865 Genome accession   NZ_CP046669
Coordinates   517517..518896 (+) Length   459 a.a.
NCBI ID   WP_003643928.1    Uniprot ID   -
Organism   Lactiplantibacillus plantarum strain 83-18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 512517..523896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPK32_RS03840 (GPK32_03840) - 512518..513210 (+) 693 WP_157262252.1 matrixin family metalloprotease -
  GPK32_RS03845 (GPK32_03845) - 513399..514730 (-) 1332 WP_003643927.1 aminopeptidase C -
  GPK32_RS03850 (GPK32_03850) rpiA 514806..515489 (-) 684 WP_003640926.1 ribose-5-phosphate isomerase RpiA -
  GPK32_RS03855 (GPK32_03855) - 515498..515794 (-) 297 WP_003640927.1 GNAT family N-acetyltransferase -
  GPK32_RS03860 (GPK32_03860) - 515933..516469 (+) 537 WP_011101073.1 dUTP diphosphatase -
  GPK32_RS03865 (GPK32_03865) radA 517517..518896 (+) 1380 WP_003643928.1 DNA repair protein RadA Machinery gene
  GPK32_RS03870 (GPK32_03870) - 518912..520075 (+) 1164 WP_046038334.1 PIN/TRAM domain-containing protein -
  GPK32_RS03875 (GPK32_03875) gltX 520399..521889 (+) 1491 WP_003640932.1 glutamate--tRNA ligase -
  GPK32_RS03880 (GPK32_03880) cysS 522167..523579 (+) 1413 WP_003644975.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50019.32 Da        Isoelectric Point: 7.7217

>NTDB_id=406538 GPK32_RS03865 WP_003643928.1 517517..518896(+) (radA) [Lactiplantibacillus plantarum strain 83-18]
MAKAKTQFVCSNCGYSTPRYLGRCPNCGEWNTLTEEHFDTKPDRKSRVSFAGKSAKPQRLKEVSLTKTPRVKTKLKEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLAETGGSVLYVTGEESASQVKMRADRLAVSSEDFYLYPETDMSNIRST
IESTHPEFVVIDSVQTMQEPDMDSAIGSVSQIREITAELLQIAKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYFEG
DLHHTYRILRAVKNRFGSTNELGIFEMRTGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALVTPTVFGN
AKRTASGLDHNRVSLIMAVLEKRASLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSVASSYRDQQTRPSDCFVGEVGLTGE
IRRVNRIESRVGEAAKLGFKRIFIPKNNLQGWDIPTGIEVIGVATLKEALQLALQQPNY

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=406538 GPK32_RS03865 WP_003643928.1 517517..518896(+) (radA) [Lactiplantibacillus plantarum strain 83-18]
GTGGCAAAAGCAAAAACTCAGTTTGTTTGTTCTAACTGTGGTTATTCAACACCGCGATATTTAGGACGGTGTCCGAACTG
TGGTGAATGGAATACACTAACTGAAGAACATTTTGATACAAAACCGGACCGCAAGTCGCGGGTGAGCTTTGCGGGTAAGT
CGGCTAAGCCACAACGACTCAAAGAAGTGTCGCTGACTAAGACGCCGCGTGTCAAGACTAAGCTGAAAGAGTTCAACCGG
GTCTTAGGTGGCGGTGTTGTGCCAGGTTCACTAGTTTTGATCGGTGGTGATCCAGGTATTGGTAAGTCAACGTTACTACT
GCAAGTTTCGGGGCAATTGGCCGAGACGGGTGGTTCCGTACTATACGTGACTGGTGAAGAAAGTGCCTCCCAAGTGAAGA
TGCGGGCGGACCGGCTGGCTGTTAGCAGTGAAGACTTTTATCTTTATCCTGAGACTGATATGTCGAATATTCGTTCAACA
ATTGAATCGACGCATCCAGAATTTGTGGTCATTGATTCGGTACAAACGATGCAAGAACCTGATATGGACTCTGCGATTGG
TTCAGTATCACAAATCCGTGAAATTACGGCTGAACTACTGCAAATTGCGAAGACTAACGGCATTACCATTTTTATTGTGG
GCCATGTGACTAAGGGTGGTGCCATTGCGGGGCCTAAGATCTTAGAACATATGGTTGATACCGTTTTGTACTTTGAAGGT
GATTTACATCATACGTACCGCATTTTACGGGCAGTCAAGAATCGATTCGGGTCAACGAATGAACTGGGAATATTTGAGAT
GCGGACGGGCGGTTTGTATGAAGTGGCCAATCCGTCAGAGATCTTCTTGGAAGAACGTCTGAAGGATGCTACGGGTTCCG
CCATCGTTGTTTCAATGGAAGGAACACGACCAATCTTAGTAGAAGTTCAAGCGCTGGTAACGCCAACGGTCTTTGGTAAT
GCGAAGCGGACCGCAAGTGGCTTGGATCATAATCGAGTCTCCTTGATTATGGCAGTGTTGGAAAAACGGGCGAGTCTAAT
GCTTCAGAACCAGGACGCGTATTTGAAAGCGGCCGGTGGCGTTAAGTTAGATGAACCGGCGATTGACTTAGCTATTGCGG
TCAGTGTGGCTTCTAGTTATCGTGACCAGCAGACTCGTCCGAGTGACTGTTTTGTTGGTGAAGTCGGTTTGACTGGTGAG
ATTCGGCGGGTCAACCGGATTGAATCACGAGTTGGTGAGGCGGCTAAGCTAGGCTTTAAACGTATCTTTATTCCTAAGAA
TAATCTGCAAGGTTGGGATATCCCAACTGGAATCGAAGTAATTGGAGTTGCCACGCTAAAAGAAGCGCTCCAATTAGCAT
TACAACAACCAAATTATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(59-220)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

67.849

98.257

0.667
  radA Streptococcus pneumoniae D39

67.849

98.257

0.667
  radA Streptococcus pneumoniae R6

67.849

98.257

0.667
  radA Streptococcus pneumoniae TIGR4

67.849

98.257

0.667
  radA Streptococcus mitis SK321

67.849

98.257

0.667
  radA Streptococcus mitis NCTC 12261

67.849

98.257

0.667
  radA Bacillus subtilis subsp. subtilis str. 168

65.57

99.346

0.651

Multiple sequence alignment