Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GPA07_RS08470 Genome accession   NZ_CP046653
Coordinates   1623916..1624986 (+) Length   356 a.a.
NCBI ID   WP_157292347.1    Uniprot ID   -
Organism   Bacillus sp. ms-22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618916..1629986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPA07_RS08430 (GPA07_08430) - 1618918..1620171 (+) 1254 WP_008360078.1 MFS transporter -
  GPA07_RS08435 (GPA07_08435) - 1620249..1620563 (+) 315 WP_008360081.1 MTH1187 family thiamine-binding protein -
  GPA07_RS08440 (GPA07_08440) - 1620588..1620761 (-) 174 WP_008360083.1 DUF2759 domain-containing protein -
  GPA07_RS08445 (GPA07_08445) - 1620919..1621557 (+) 639 WP_008360086.1 MBL fold metallo-hydrolase -
  GPA07_RS08450 (GPA07_08450) - 1621806..1622048 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  GPA07_RS08455 (GPA07_08455) - 1622260..1622640 (+) 381 WP_071168934.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GPA07_RS08465 (GPA07_08465) - 1622866..1623738 (+) 873 WP_008360222.1 STAS domain-containing protein -
  GPA07_RS08470 (GPA07_08470) comGA 1623916..1624986 (+) 1071 WP_157292347.1 competence type IV pilus ATPase ComGA Machinery gene
  GPA07_RS08475 (GPA07_08475) comGB 1624967..1626007 (+) 1041 WP_157292348.1 competence type IV pilus assembly protein ComGB -
  GPA07_RS08480 (GPA07_08480) comGC 1626024..1626317 (+) 294 WP_008360227.1 competence type IV pilus major pilin ComGC Machinery gene
  GPA07_RS08485 (GPA07_08485) comGD 1626310..1626756 (+) 447 WP_008360229.1 competence type IV pilus minor pilin ComGD -
  GPA07_RS08490 (GPA07_08490) comGE 1626740..1627057 (+) 318 WP_008360231.1 competence type IV pilus minor pilin ComGE -
  GPA07_RS08495 (GPA07_08495) comGF 1626966..1627496 (+) 531 WP_232532127.1 competence type IV pilus minor pilin ComGF -
  GPA07_RS08500 (GPA07_08500) comGG 1627493..1627876 (+) 384 WP_008360235.1 competence type IV pilus minor pilin ComGG -
  GPA07_RS08505 (GPA07_08505) - 1627937..1628125 (+) 189 WP_008360237.1 YqzE family protein -
  GPA07_RS08510 (GPA07_08510) - 1628159..1628485 (-) 327 WP_008360239.1 YqzG/YhdC family protein -
  GPA07_RS08515 (GPA07_08515) tapA 1628744..1629276 (+) 533 Protein_1653 amyloid fiber anchoring/assembly protein TapA -
  GPA07_RS08520 (GPA07_08520) - 1629324..1629896 (+) 573 WP_008360243.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40296.64 Da        Isoelectric Point: 9.1842

>NTDB_id=406426 GPA07_RS08470 WP_157292347.1 1623916..1624986(+) (comGA) [Bacillus sp. ms-22]
MYGIEYVGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRTGQVFVRMSTLPTVNDESLVIRILPQDHVPKTKYLSLYPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYATGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAITAQRLIELTCPFCGETCELYCKLNRPVRRTNVFELLYGKKLGECVKEAKGEY
AHSSYETLQKLIRKGVALGYLSKNTYHRWVYEEAGI

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=406426 GPA07_RS08470 WP_157292347.1 1623916..1624986(+) (comGA) [Bacillus sp. ms-22]
TTGTATGGTATTGAATATGTGGGACAAGAGCTGCTAGAAGAAGCTTGCCGCATGAGAGCATCTGATGTGCATATTGTACC
AAAAGAAAAGGAAGCTTCGGTATCTTTCCGTGTAGACTCAGATTTAATCCAGCAGCGAACCATCGATAAAAAAAGCGGGG
AGCGGTTAATTGCTCATTTTAAATTTTTATCCTCCATGGATATCGGTGAAAAAAGGAGACCGCAAAATGGGTCATTGGCT
GTGATGCTAAGAACTGGACAAGTGTTTGTACGGATGTCTACATTACCGACTGTGAATGATGAAAGTTTAGTGATTAGAAT
TTTACCGCAGGATCATGTCCCAAAAACAAAATATCTGTCGCTATATCCTAAAGCTTCAGCCAGATTATTATCATTTTTAA
ATCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTTTATTCATTGATACAGTTT
GCAAAAAAGCATTTCAATCGAAATATTATTACACTTGAGGACCCTGTGGAAACAAGAAATGAAGAAGTGCTACAGGTACA
AGTGAATGAAAAAGCAGGTATTACGTATGCGACAGGATTACGTGCTATTTTAAGACACGACCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACAGCAAGAACAGCAATAAGAGCAGCACTGACAGGTCATTTAGTGATGAGTACGCTGCAT
GCGAAAAATGCAAAAGGTGCCCTTTACCGAATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACCATGGTTGC
GATTACTGCGCAGCGTTTAATCGAGCTCACATGTCCTTTTTGCGGGGAAACGTGTGAGCTTTATTGCAAATTAAATCGCC
CGGTCAGGCGAACGAATGTGTTTGAATTGCTATACGGAAAGAAGCTTGGTGAGTGTGTCAAAGAGGCCAAAGGAGAATAT
GCTCACTCGTCATATGAAACATTGCAAAAATTAATTCGTAAAGGAGTGGCACTGGGCTATCTATCGAAAAACACATATCA
TCGCTGGGTATATGAAGAAGCAGGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment