Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GOQ09_RS02530 Genome accession   NZ_CP046622
Coordinates   542589..544184 (+) Length   531 a.a.
NCBI ID   WP_157611741.1    Uniprot ID   A0A6I6H583
Organism   Variovorax paradoxus strain CSUSB     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537589..549184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOQ09_RS02505 (GOQ09_02505) argS 537658..539343 (+) 1686 WP_157611736.1 arginine--tRNA ligase -
  GOQ09_RS02510 (GOQ09_02510) - 539351..540097 (+) 747 WP_157611737.1 SPOR domain-containing protein -
  GOQ09_RS02515 (GOQ09_02515) - 540262..540909 (+) 648 WP_157611738.1 thiol:disulfide interchange protein DsbA/DsbL -
  GOQ09_RS02520 (GOQ09_02520) lptA 541061..541780 (+) 720 WP_157611739.1 lipopolysaccharide transport periplasmic protein LptA -
  GOQ09_RS02525 (GOQ09_02525) lptB 541777..542571 (+) 795 WP_157611740.1 LPS export ABC transporter ATP-binding protein -
  GOQ09_RS02530 (GOQ09_02530) rpoN/rpoN1 542589..544184 (+) 1596 WP_157611741.1 RNA polymerase factor sigma-54 Machinery gene
  GOQ09_RS02535 (GOQ09_02535) - 544212..544853 (-) 642 WP_157611742.1 LysE family translocator -
  GOQ09_RS02540 (GOQ09_02540) - 544958..546208 (+) 1251 WP_157611743.1 THUMP domain-containing protein -
  GOQ09_RS02545 (GOQ09_02545) - 546228..546695 (-) 468 WP_242630965.1 surface-adhesin E family protein -
  GOQ09_RS02550 (GOQ09_02550) - 546701..547216 (+) 516 WP_157611744.1 putative toxin-antitoxin system toxin component, PIN family -
  GOQ09_RS02555 (GOQ09_02555) - 547221..548144 (-) 924 WP_157611745.1 hypothetical protein -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 58606.97 Da        Isoelectric Point: 4.7559

>NTDB_id=406112 GOQ09_RS02530 WP_157611741.1 542589..544184(+) (rpoN/rpoN1) [Variovorax paradoxus strain CSUSB]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPAPRDEAAEAGEGE
FASLPASSATTSASETGTSTSDTETPAADIAEREPDWEGDGTVDMAPDDSEWGGDAPARQNNLGDDERADATELARSQES
LQSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPTGVGARD
LGECLSIQLRALASKDETEEQALVRKTAIAICKQPMELLARRDFKRLATLTRTNEDLVRSALQIISRLEPKPGRRFVDVE
RNIVIPDVIVTKIGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSN
AIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIK
QFVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=406112 GOQ09_RS02530 WP_157611741.1 542589..544184(+) (rpoN/rpoN1) [Variovorax paradoxus strain CSUSB]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTTCA
GCTCTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTCGACGAAAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTCGACGCCGTCGACACCCCCGCGCCGCGCGATGAAGCGGCGGAGGCCGGCGAGGGCGAA
TTCGCCTCGCTGCCGGCCAGCAGCGCGACCACCTCTGCGTCCGAGACAGGCACCTCCACAAGCGACACCGAAACCCCCGC
CGCCGACATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCG
GCGATGCGCCGGCGCGGCAGAACAACCTTGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAGTCG
CTGCAATCCTTTCTGCACCGGCAGGCGCTCAGCCTGCGCCTGAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGA
GTCGCTCAACGATGACGGCTATCTTGAAGATTCGCTGCCTGCCCTGGCCTCGGGCCTTGCGGGCGACGACAATGACCAGT
TCGACGAACTGGTCCACCACTTCCAGGTGGCACTCGGCCTGCTGCAGAGCCTGGAGCCCACCGGTGTCGGCGCGCGCGAC
CTGGGCGAATGCCTGAGCATCCAGCTGCGCGCGCTGGCCAGCAAGGACGAAACCGAAGAACAGGCGCTGGTCCGCAAGAC
CGCCATCGCCATCTGCAAGCAACCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCTTGGCCACGCTCACCCGCACCA
ACGAAGACCTGGTGCGCTCGGCGCTGCAGATCATTTCGCGCCTGGAGCCCAAGCCGGGGCGCCGCTTTGTCGACGTCGAG
CGCAACATCGTGATCCCCGACGTCATCGTCACCAAGATCGGCCGCGGCACCAACGTCAAGTTCCGCGTCATGCTCAACCC
CGAGGTGATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAAGGCAGCCAGGCGC
TGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAAC
GCCATCGTCGAGCGGCAGAAGAGCTACTTCGTGCACGGCGAGCTTGCCATGCGCCCGCTGGTGCTGCGCGAGATTGCCGA
CGAGCTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGGCTACGCCGTTCGGCACGGTCGAGC
TCAAGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCCGTGCGCGCGCTGATCAAG
CAGTTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATTTCCGAAATGCTGAAAGAGCAGGGCATCGA
ATGCGCACGCCGCACCGTGGCCAAGTACCGCGAAGCGTTGCGCATCGCGCCGGCCAACCTGCGCAAGGCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(373-529)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-359)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6H583

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.504

100

0.516

Multiple sequence alignment