Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PT7_RS14950 Genome accession   NC_015458
Coordinates   3242840..3244252 (+) Length   470 a.a.
NCBI ID   WP_049790334.1    Uniprot ID   -
Organism   Pusillimonas sp. T7-7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3237840..3249252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT7_RS14930 (PT7_3060) - 3238192..3238986 (-) 795 WP_013744124.1 phosphodiesterase -
  PT7_RS14935 (PT7_3061) - 3238989..3240038 (-) 1050 WP_013744125.1 LacI family DNA-binding transcriptional regulator -
  PT7_RS14940 (PT7_3062) leuA 3240253..3241956 (-) 1704 WP_013744126.1 2-isopropylmalate synthase -
  PT7_RS14945 (PT7_3063) - 3242251..3242823 (-) 573 WP_013744127.1 hypothetical protein -
  PT7_RS14950 (PT7_3064) rpoN/rpoN1 3242840..3244252 (+) 1413 WP_049790334.1 RNA polymerase factor sigma-54 Machinery gene
  PT7_RS14955 (PT7_3065) - 3244363..3244626 (+) 264 WP_013744129.1 (2Fe-2S)-binding protein -
  PT7_RS14960 (PT7_3066) - 3244629..3244883 (-) 255 WP_013744130.1 hypothetical protein -
  PT7_RS14965 (PT7_3067) bfr 3245015..3245491 (+) 477 WP_013744131.1 bacterioferritin -
  PT7_RS14970 (PT7_3068) - 3245508..3246161 (-) 654 WP_013744132.1 hypothetical protein -
  PT7_RS14975 (PT7_3069) - 3246279..3247886 (-) 1608 WP_013744133.1 peptide chain release factor 3 -
  PT7_RS14980 (PT7_3070) - 3247890..3248519 (-) 630 WP_013744134.1 LysE family transporter -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 52322.55 Da        Isoelectric Point: 5.1235

>NTDB_id=40604 PT7_RS14950 WP_049790334.1 3242840..3244252(+) (rpoN/rpoN1) [Pusillimonas sp. T7-7]
MQSRQSLELRQHQQLALTPQLQQSIRFLQLSTHELEQEVAQALQDNPLLEREEEYDTDAGDAVVDHAQALEERWTVLGLS
NRSSNNNADDEPQRPEAALMETLQEHLQEQLRLTRAQPRDCALVSLLIDELDENGYLPTSLDDILTFLPPELVVDPDELY
AALRLLQSFDPPGVGARSLAECLCLQLNHLQALADTAALPHDVLALARRIAVDHLALLATGNLNRLRDALACDHELLLSA
HALLLQLEPKPAKDWATSTADYITPDVLVRKMGGVWMVTVNPAIMPRLRINRVYESLLSTSPPAPAMQTQLQQAHGLIKS
VNQRFVTIQRVMQAIVDRQKDFFEQGHSGLRPMQLRDIAQELELHESTISRATKQKYAQTPWGVIELKRFFSSALQTDDG
QSTTASAVQVLIQKLVGQESGKKPLSDSQIAAQLADQGVVIARRTVAKYREAAGIEPAIIRKARAALEAR

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=40604 PT7_RS14950 WP_049790334.1 3242840..3244252(+) (rpoN/rpoN1) [Pusillimonas sp. T7-7]
ATGCAATCGCGTCAGTCCCTAGAGCTTCGCCAACATCAGCAATTGGCTTTGACCCCCCAACTGCAGCAATCGATTCGATT
TTTGCAGTTGTCGACGCACGAGCTGGAACAGGAGGTGGCGCAGGCATTGCAAGATAACCCGCTGCTTGAACGTGAGGAAG
AGTATGACACCGATGCTGGCGATGCGGTAGTCGATCACGCACAAGCACTGGAAGAGCGCTGGACGGTGTTGGGTTTATCC
AACCGCTCTTCCAACAACAACGCCGATGACGAACCGCAACGCCCCGAGGCGGCCTTGATGGAAACCCTGCAGGAGCATTT
GCAGGAACAATTGCGCCTGACGCGCGCGCAGCCACGCGACTGCGCCCTGGTGTCTTTGCTGATCGATGAGCTGGACGAAA
ACGGCTATTTGCCCACCAGCCTGGATGACATTCTGACTTTCCTGCCGCCCGAGCTGGTGGTGGACCCAGATGAGTTGTAT
GCGGCACTACGGCTCTTGCAGTCATTCGATCCGCCGGGCGTGGGGGCGCGTTCTCTGGCGGAATGCCTTTGTTTGCAGTT
GAATCATTTGCAGGCGCTGGCCGACACCGCAGCATTGCCCCACGATGTGCTGGCTCTGGCCCGACGGATTGCTGTCGACC
ATTTGGCCTTGCTGGCCACGGGCAACCTGAATCGGCTGCGTGATGCCTTGGCCTGCGACCACGAGTTGTTGCTGTCGGCA
CATGCCTTGTTGCTGCAGCTGGAGCCCAAACCGGCAAAAGATTGGGCCACCTCGACTGCTGACTACATCACCCCCGATGT
ACTGGTACGCAAAATGGGTGGCGTATGGATGGTCACGGTCAACCCGGCCATCATGCCGCGCCTGCGCATCAACAGGGTTT
ATGAAAGCCTGTTGAGCACCTCCCCCCCAGCGCCCGCCATGCAAACACAGCTGCAGCAGGCGCATGGCCTGATCAAAAGC
GTGAACCAGCGCTTCGTGACCATACAACGGGTGATGCAGGCCATCGTAGACCGGCAAAAAGACTTTTTTGAGCAGGGACA
CAGCGGGCTGCGCCCCATGCAACTGCGCGACATCGCCCAGGAACTGGAGCTGCATGAATCCACCATATCGCGCGCAACCA
AGCAAAAGTACGCACAAACGCCCTGGGGCGTCATTGAACTGAAGCGTTTCTTTTCCAGCGCACTGCAAACTGACGATGGC
CAGAGCACCACGGCATCCGCGGTGCAGGTGCTGATTCAGAAGTTGGTGGGTCAAGAATCGGGTAAGAAGCCGTTGTCGGA
TAGTCAGATTGCTGCCCAATTGGCGGATCAAGGTGTCGTCATCGCGCGCCGAACAGTAGCCAAATATCGTGAAGCTGCCG
GCATCGAGCCGGCTATTATCCGCAAGGCACGTGCTGCGCTTGAAGCGCGTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(307-462)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(98-294)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.337

100

0.511

Multiple sequence alignment