Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PT7_RS02335 Genome accession   NC_015458
Coordinates   561336..562697 (+) Length   453 a.a.
NCBI ID   WP_041682920.1    Uniprot ID   -
Organism   Pusillimonas sp. T7-7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 556336..567697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT7_RS02315 (PT7_0478) hpnD 557477..558340 (-) 864 WP_013741567.1 presqualene diphosphate synthase HpnD -
  PT7_RS02320 (PT7_0479) hpnC 558386..559270 (-) 885 WP_041682919.1 squalene synthase HpnC -
  PT7_RS02325 (PT7_0480) alr 559359..560489 (+) 1131 WP_013741569.1 alanine racemase -
  PT7_RS02330 (PT7_0481) - 560593..561309 (+) 717 WP_013741570.1 SDR family oxidoreductase -
  PT7_RS02335 (PT7_0482) radA 561336..562697 (+) 1362 WP_041682920.1 DNA repair protein RadA Machinery gene
  PT7_RS02340 (PT7_0483) - 562707..563483 (+) 777 WP_013741572.1 sulfite exporter TauE/SafE family protein -
  PT7_RS02345 (PT7_0484) - 563489..565990 (+) 2502 WP_013741573.1 ABC transporter permease -
  PT7_RS02350 (PT7_0485) - 565990..566706 (+) 717 WP_013741574.1 3-deoxy-D-manno-octulosonic acid kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 47989.39 Da        Isoelectric Point: 7.1656

>NTDB_id=40593 PT7_RS02335 WP_041682920.1 561336..562697(+) (radA) [Pusillimonas sp. T7-7]
MAKSKTLFICSECGGSCPKWEGKCPHCGSWNTLQESRDTGVPAHRYAPLAGASPVRSLAEIEARELPRQPSGIAEFDRVL
GGGLVSGAVVLIGGDPGIGKSTLLLQALVSLSRAVKVLYVTGEESAEQVALRARRLELVTGEVDLLAEIRLEAIMAALAE
QQPSVAVIDSIQTLYSSELSAAPGSVSQVRECAAQLTRLAKQTGTTIVLIGHVTKDGTLAGPRVLEHIVDTVLYFEGDTH
SSFRLVRAFKNRFGAVNELGVFAMTDRGLRGVANPSALFLSQHEQNVPGSCVMATQEGTRPLLVEIQALVDTAHVPNPRR
LSVGLEGTRLAMLLAVLHRHAGVVTFDQDVFVNAVGGVKITEPAADLAVLLAIMSSLGNKPLPKGLIVFGEVGLAGEIRP
APRGQERLREAAKLGFSVALIPKANAPKQAIEGLEIWAVDRVDAAIARVREAS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=40593 PT7_RS02335 WP_041682920.1 561336..562697(+) (radA) [Pusillimonas sp. T7-7]
ATGGCCAAGTCAAAAACCCTGTTTATCTGTAGCGAGTGCGGCGGAAGCTGCCCCAAGTGGGAAGGAAAATGTCCGCACTG
CGGATCCTGGAATACGCTTCAGGAATCACGTGACACCGGCGTGCCTGCGCACCGCTATGCACCTTTGGCGGGGGCCAGCC
CGGTGCGCAGCCTGGCTGAAATCGAAGCGCGCGAGCTGCCTCGCCAGCCCTCGGGTATTGCCGAGTTCGACCGGGTGCTG
GGCGGCGGGCTGGTGTCGGGCGCTGTCGTCCTGATTGGCGGTGATCCGGGCATAGGCAAGTCCACCCTGCTTTTGCAGGC
TCTGGTGTCCTTGTCCCGTGCCGTCAAGGTTTTATATGTGACAGGCGAAGAGTCGGCCGAGCAGGTCGCCTTGCGTGCCA
GACGGCTGGAGCTGGTAACAGGCGAGGTCGATCTGCTGGCGGAAATCCGCCTGGAGGCCATTATGGCCGCGCTGGCTGAA
CAGCAACCCAGCGTTGCCGTGATCGACTCCATCCAAACACTTTATTCCAGTGAACTCAGCGCTGCTCCCGGGTCGGTCTC
TCAGGTGCGCGAGTGTGCAGCGCAGCTTACCCGGCTGGCCAAGCAGACGGGCACTACCATTGTACTGATCGGCCACGTCA
CCAAAGACGGCACGCTGGCCGGGCCGCGAGTACTGGAACATATCGTTGACACCGTCCTGTATTTCGAGGGCGACACCCAC
TCATCCTTTCGCCTGGTCAGGGCGTTCAAGAATCGCTTTGGAGCCGTCAATGAGCTGGGCGTATTCGCCATGACTGATCG
CGGTCTGCGTGGCGTAGCCAATCCTTCCGCCCTGTTCCTGTCACAACACGAGCAGAACGTGCCGGGTTCCTGCGTCATGG
CTACGCAAGAAGGCACGAGGCCTTTGCTGGTCGAGATCCAGGCGCTGGTAGATACCGCACACGTACCCAATCCCCGGCGC
TTGTCGGTAGGTCTTGAAGGCACGCGCCTGGCGATGCTGCTGGCTGTGCTGCACAGGCATGCGGGCGTGGTCACCTTTGA
CCAAGATGTATTCGTCAATGCTGTCGGTGGTGTCAAGATTACCGAGCCCGCGGCCGATCTGGCGGTCTTGCTGGCCATTA
TGTCGTCCTTGGGAAACAAGCCCTTGCCCAAAGGCCTGATCGTATTCGGCGAGGTAGGCCTGGCCGGCGAGATCAGGCCT
GCCCCGAGGGGCCAGGAAAGGCTGCGCGAGGCGGCCAAGCTGGGCTTCAGCGTGGCGCTCATTCCCAAGGCGAATGCACC
CAAGCAGGCTATTGAAGGTCTGGAAATCTGGGCGGTGGATCGTGTTGATGCGGCCATCGCGCGTGTGCGCGAAGCTTCAT
AG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(356-446)

AAA domain (AAA_25)

(78-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.802

100

0.494
  radA Streptococcus mitis SK321

48.941

93.819

0.459
  radA Streptococcus pneumoniae Rx1

48.941

93.819

0.459
  radA Streptococcus pneumoniae D39

48.941

93.819

0.459
  radA Streptococcus pneumoniae R6

48.941

93.819

0.459
  radA Streptococcus pneumoniae TIGR4

48.941

93.819

0.459
  radA Streptococcus mitis NCTC 12261

48.706

93.819

0.457

Multiple sequence alignment