Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GOY15_RS09160 Genome accession   NZ_CP046589
Coordinates   1850179..1851555 (-) Length   458 a.a.
NCBI ID   WP_005720413.1    Uniprot ID   A0A2A7RGN6
Organism   Lactobacillus crispatus strain M247_Siena     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1845179..1856555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOY15_RS09135 (GOY15_09135) - 1845221..1845766 (-) 546 WP_005720419.1 DNA-directed RNA polymerase subunit sigma -
  GOY15_RS09140 (GOY15_09140) rlmB 1845900..1846655 (-) 756 WP_005720418.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GOY15_RS09145 (GOY15_09145) - 1846642..1847085 (-) 444 WP_005720417.1 mini-ribonuclease III -
  GOY15_RS09150 (GOY15_09150) cysS 1847078..1848502 (-) 1425 WP_419389272.1 cysteine--tRNA ligase -
  GOY15_RS09155 (GOY15_09155) gltX 1848602..1850101 (-) 1500 WP_005720415.1 glutamate--tRNA ligase -
  GOY15_RS09160 (GOY15_09160) radA 1850179..1851555 (-) 1377 WP_005720413.1 DNA repair protein RadA Machinery gene
  GOY15_RS09165 (GOY15_09165) - 1851555..1852106 (-) 552 WP_005720412.1 dUTP diphosphatase -
  GOY15_RS09170 (GOY15_09170) - 1852276..1853310 (-) 1035 WP_005729050.1 IS30 family transposase -
  GOY15_RS09175 (GOY15_09175) - 1853417..1853716 (+) 300 WP_005721602.1 hypothetical protein -
  GOY15_RS09180 (GOY15_09180) pepC 1853784..1855133 (+) 1350 WP_005720410.1 aminopeptidase C -
  GOY15_RS09185 (GOY15_09185) - 1855273..1855677 (+) 405 WP_005720409.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50214.83 Da        Isoelectric Point: 7.1405

>NTDB_id=405721 GOY15_RS09160 WP_005720413.1 1850179..1851555(-) (radA) [Lactobacillus crispatus strain M247_Siena]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMYSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=405721 GOY15_RS09160 WP_005720413.1 1850179..1851555(-) (radA) [Lactobacillus crispatus strain M247_Siena]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGTTATTTAGGCCGCTGTCCTAACTG
TGGTGCCTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTGATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAACCGCGTTTTAGGTGGTGGTATTGTACCAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTAATCGACTCCATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGGCCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAATTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGTATTCTGGATTAAAAGATCATGGCATTGAAGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A7RGN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559

Multiple sequence alignment