Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GOY15_RS00985 Genome accession   NZ_CP046589
Coordinates   180526..182322 (-) Length   598 a.a.
NCBI ID   WP_005718172.1    Uniprot ID   A0A135YP31
Organism   Lactobacillus crispatus strain M247_Siena     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 175526..187322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOY15_RS00930 (GOY15_00930) - 175749..175946 (+) 198 WP_005723817.1 hypothetical protein -
  GOY15_RS00935 (GOY15_00935) - 175968..176243 (+) 276 WP_005723819.1 bacteriocin immunity protein -
  GOY15_RS00940 (GOY15_00940) - 176546..176692 (+) 147 WP_005727423.1 hypothetical protein -
  GOY15_RS00945 (GOY15_00945) - 176702..176848 (+) 147 WP_005723821.1 hypothetical protein -
  GOY15_RS00950 (GOY15_00950) - 177026..178204 (-) 1179 WP_419389271.1 IS256 family transposase -
  GOY15_RS00955 (GOY15_00955) - 178391..178651 (+) 261 WP_005726357.1 thioredoxin domain-containing protein -
  GOY15_RS00960 (GOY15_00960) - 178667..178963 (+) 297 WP_005725464.1 bacteriocin immunity protein -
  GOY15_RS00965 (GOY15_00965) - 179137..179355 (+) 219 WP_005726358.1 TIGR04139 family peptide modification target -
  GOY15_RS00970 (GOY15_00970) - 179359..179634 (+) 276 WP_005723823.1 bacteriocin immunity protein -
  GOY15_RS00975 (GOY15_00975) - 179748..180155 (-) 408 WP_005723824.1 type II toxin-antitoxin system death-on-curing family toxin -
  GOY15_RS00980 (GOY15_00980) - 180159..180374 (-) 216 WP_005723825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  GOY15_RS00985 (GOY15_00985) pepF 180526..182322 (-) 1797 WP_005718172.1 oligoendopeptidase F Regulator
  GOY15_RS00990 (GOY15_00990) - 182490..183728 (+) 1239 WP_005718173.1 LCP family protein -
  GOY15_RS00995 (GOY15_00995) - 183793..184482 (+) 690 WP_005718174.1 DUF554 domain-containing protein -
  GOY15_RS01000 (GOY15_01000) - 184479..184862 (-) 384 WP_005718176.1 PBECR4 domain-containing protein -
  GOY15_RS01005 (GOY15_01005) - 185352..185903 (-) 552 WP_005722451.1 GNAT family N-acetyltransferase -
  GOY15_RS01010 (GOY15_01010) - 186048..186425 (+) 378 WP_005718180.1 hypothetical protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68032.88 Da        Isoelectric Point: 4.6146

>NTDB_id=405713 GOY15_RS00985 WP_005718172.1 180526..182322(-) (pepF) [Lactobacillus crispatus strain M247_Siena]
MAIPNRNEVPEELKWDLTRIFKNDEEWEQAYAAAQEKIDQLGELKGTLAKSGKDLYEGLTKILAVKREVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLAEFKQAEPRLKNYAHYLEMITNKRPHTLPAEQEKLIAD
AGDALGVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYSQFQNSLASTLSGVVKK
HNYNAKAHKYDSARQAALAENGVPEAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPSLSYNFEEA
KEVAKKALKPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADANGEPLTA
DTLDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=405713 GOY15_RS00985 WP_005718172.1 180526..182322(-) (pepF) [Lactobacillus crispatus strain M247_Siena]
ATGGCGATACCAAACAGAAATGAAGTCCCAGAAGAACTTAAGTGGGACTTGACCCGAATTTTTAAAAATGATGAAGAATG
GGAACAGGCCTATGCAGCGGCCCAAGAAAAAATCGACCAACTTGGTGAATTAAAAGGTACATTAGCAAAATCTGGCAAAG
ATTTGTACGAAGGCTTAACTAAGATTCTGGCCGTTAAGCGCGAGGTGGAAAATATTTATGTTTATGCCACCATGTCTAGC
GATGTGGATACTGCCAATTCACATTATTTAGGCTATGTCAGCCGGGTGCAAAGCTTAGCTAACCAATTCGAAGCCGCTAC
TAGTTTTATTAACCCAGAAATCTTGAGCATCCCTAGTGATAAACTAGCTGAATTTAAGCAAGCTGAGCCAAGACTAAAGA
ATTATGCTCACTACTTGGAAATGATTACTAATAAGCGACCTCATACCCTGCCAGCTGAACAAGAAAAGTTAATCGCTGAT
GCTGGAGATGCTTTAGGCGTTTCCGAAAATACTTTTAACGTTTTAACTAACTCTGACATGGAATACGGCTACGTCCAAGA
TGACGATGGCAACATGGAACAACTATCTGACGGTTTGTACTCATTATTAATTCAATCGCAAAACCGTGACGTCAGAAAAG
GTGCCTTCGACACTCTCTATGCTACGTACAGCCAATTCCAGAATTCGCTGGCTTCAACCTTGTCCGGCGTAGTGAAAAAA
CACAACTATAACGCTAAAGCGCACAAATATGACTCTGCCCGTCAAGCAGCACTGGCTGAAAACGGCGTACCTGAAGCAGT
TTACGATACTTTAATTCAAGAAGTTGATTCTCACCTTGACCTGCTCCATCGTTACGTTGCCTTGCGCAAGAAAATTTTAG
GCTTAAAAGATTTGCAAATGTGGGACATGTACGTGCCGCTAACTGGGAAGCCTTCTCTTTCTTATAACTTTGAAGAAGCT
AAGGAAGTTGCTAAAAAGGCATTGAAGCCTTTGGGCGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAACAAGGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCCGATGCTAATGGAGAACCACTGACCGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCGATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACAGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGCTTTCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGTGCTGGCGTAGACATGACTAAGCCTGACTACTTAGAAGATGCCTTCAAGACTTTTGAAAA
ACGGTTGAACGAATTTGAAAGCTTGATTGAGAAATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A135YP31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.515

99.331

0.512

Multiple sequence alignment