Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GN206_RS11435 Genome accession   NZ_CP046558
Coordinates   2259412..2260794 (+) Length   460 a.a.
NCBI ID   WP_006121838.1    Uniprot ID   A0A9Q6N3S3
Organism   Pantoea stewartii subsp. stewartii strain ATCC 8199     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2254412..2265794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN206_RS11405 (GN206_11285) rsmC 2254872..2255903 (-) 1032 WP_006121832.1 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC -
  GN206_RS11410 (GN206_11290) - 2256009..2256419 (+) 411 WP_006121833.1 DNA polymerase III subunit psi -
  GN206_RS11415 (GN206_11295) rimI 2256391..2256831 (+) 441 WP_006121834.1 ribosomal protein S18-alanine N-acetyltransferase -
  GN206_RS11420 (GN206_11300) yjjG 2256851..2257531 (+) 681 WP_006121835.1 pyrimidine 5'-nucleotidase -
  GN206_RS11425 (GN206_11305) - 2257645..2258313 (-) 669 WP_006121836.1 YtjB family periplasmic protein -
  GN206_RS11430 (GN206_11310) serB 2258421..2259398 (+) 978 WP_006121837.1 phosphoserine phosphatase -
  GN206_RS11435 (GN206_11315) radA 2259412..2260794 (+) 1383 WP_006121838.1 DNA repair protein RadA Machinery gene
  GN206_RS11440 (GN206_11320) nadR 2260887..2262125 (+) 1239 WP_006121839.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  GN206_RS11445 (GN206_11325) - 2262384..2263352 (-) 969 WP_377776228.1 IS5 family transposase -
  GN206_RS11450 (GN206_11330) - 2263695..2264612 (+) 918 WP_006121841.1 winged helix-turn-helix domain-containing protein -
  GN206_RS11455 (GN206_11335) - 2264612..2265061 (+) 450 WP_006121842.1 hypothetical protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49464.08 Da        Isoelectric Point: 7.4215

>NTDB_id=405515 GN206_RS11435 WP_006121838.1 2259412..2260794(+) (radA) [Pantoea stewartii subsp. stewartii strain ATCC 8199]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGNAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVMCRLAESMKTLYVTGEESLQQVAMRAHRLGLPSDNLNMLSETSIE
QICLIAEQEQPQIMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEITSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERIAEAAKHGFKRAIVPAGNAPKKPIEGMRVYSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=405515 GN206_RS11435 WP_006121838.1 2259412..2260794(+) (radA) [Pantoea stewartii subsp. stewartii strain ATCC 8199]
TTGGCCAAAGCGGCAAAACGCGCATTTGTCTGTAACGAGTGCGGCGCAGATTATCCACGCTGGCAGGGGCAGTGCAGCGC
CTGTCACGCCTGGAACACGATTACCGAGGTGCGTATTGCGGCCTCACCGGCGGCGGCGCGCAATGAGCGCCTCAGCGGTT
ATGCAGGCAATGCCGGAACCAGTCGGGTGCAAAAGCTGTCCGAAATCAGTCTGGAAGCCTTGCCCCGTTTTTCGACCAGT
TTTAAAGAGTTTGACCGCGTGCTGGGTGGCGGTGTGGTGCCGGGCAGTGCCATTCTGATTGGCGGTAACCCCGGCGCCGG
GAAATCGACGCTGCTGTTGCAGGTGATGTGCCGTTTAGCCGAGAGCATGAAAACCCTCTACGTCACCGGTGAGGAGTCAT
TACAGCAGGTCGCTATGCGCGCCCATCGTCTGGGATTGCCGAGTGACAATCTGAACATGCTGTCGGAAACCAGCATTGAG
CAGATCTGCCTGATTGCGGAGCAGGAGCAGCCGCAAATCATGGTGATCGACTCGATTCAGGTGATGCACATGGCGGATAT
CCAGTCGTCGCCGGGCAGCGTGGCGCAGGTGCGTGAAACGGCCGCCTATCTGACGCGTTTCGCTAAAACCCGTGGCGTGG
CAATTATCATGGTGGGCCATGTGACCAAAGACGGATCGCTGGCCGGGCCAAAAGTTCTGGAGCACTGCATTGACTGCTCC
GTTCTGCTGGACGGCGATGCCGATTCCCGCTTCCGGACGTTACGTAGCCATAAAAACCGCTTTGGTGCCGTCAATGAACT
TGGCGTATTTGCCATGACCGAGCAGGGCATGCGTGAGGTGAGCAACCCGTCCGCCATTTTCCTGTCGCGTGGTGAAGAGA
TCACGTCCGGCAGTTCGGTCATGGTGTTATGGGAAGGCACCCGTCCTTTACTGGTGGAGATTCAGGCGCTGGTGGATCAT
TCGATGATGGGCAATCCGCGTCGGGTTGCGGTCGGTCTGGAACAGAACCGCCTGGCCATTCTGCTGGCAGTGCTGCATCG
TCATGGCGGTTTGCAAATGGCGGATCAGGATGTGTTCGTCAACGTGGTCGGCGGCGTGAAAGTCACGGAAACCAGTGCTG
ACCTGGCCCTGATGCTGTCGATGGTGTCCAGCCTGCGCGATCGCCCTTTGCCGCAGGATCTGGTTATCTTTGGCGAGGTC
GGACTGGCCGGAGAGATTCGTCCGGTGCCCAGCGGACAGGAGCGCATTGCTGAAGCGGCTAAACATGGCTTTAAGCGGGC
GATCGTGCCGGCGGGTAACGCACCGAAAAAGCCGATTGAGGGCATGCGCGTATACAGCGCGAAGAAGCTGGCGGATGCGT
TGGCTATCCTTGATGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(87-225)

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.59

100

0.487
  radA Streptococcus mitis NCTC 12261

43.736

98.913

0.433
  radA Streptococcus pneumoniae Rx1

45.012

93.696

0.422
  radA Streptococcus pneumoniae D39

45.012

93.696

0.422
  radA Streptococcus pneumoniae R6

45.012

93.696

0.422
  radA Streptococcus pneumoniae TIGR4

45.012

93.696

0.422
  radA Streptococcus mitis SK321

45.012

93.696

0.422

Multiple sequence alignment