Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GNG29_RS20360 Genome accession   NZ_CP046557
Coordinates   4269556..4270989 (+) Length   477 a.a.
NCBI ID   WP_103177192.1    Uniprot ID   -
Organism   Leclercia sp. Colony189 strain 1106151     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264556..4275989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNG29_RS20330 (GNG29_20330) - 4265123..4266100 (+) 978 WP_103177187.1 calcium/sodium antiporter -
  GNG29_RS20335 (GNG29_20335) kdsD 4266114..4267100 (+) 987 WP_156655276.1 arabinose-5-phosphate isomerase KdsD -
  GNG29_RS20340 (GNG29_20340) kdsC 4267115..4267681 (+) 567 WP_103824984.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GNG29_RS20345 (GNG29_20345) lptC 4267678..4268253 (+) 576 WP_103177189.1 LPS export ABC transporter periplasmic protein LptC -
  GNG29_RS20350 (GNG29_20350) lptA 4268222..4268776 (+) 555 WP_103177190.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  GNG29_RS20355 (GNG29_20355) lptB 4268783..4269508 (+) 726 WP_103177191.1 LPS export ABC transporter ATP-binding protein -
  GNG29_RS20360 (GNG29_20360) rpoN/rpoN1 4269556..4270989 (+) 1434 WP_103177192.1 RNA polymerase factor sigma-54 Machinery gene
  GNG29_RS20365 (GNG29_20365) hpf 4271012..4271299 (+) 288 WP_103177193.1 ribosome hibernation promoting factor -
  GNG29_RS20370 (GNG29_20370) ptsN 4271382..4271873 (+) 492 WP_103177194.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GNG29_RS20375 (GNG29_20375) rapZ 4271919..4272773 (+) 855 WP_032614487.1 RNase adapter RapZ -
  GNG29_RS20380 (GNG29_20380) npr 4272770..4273042 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  GNG29_RS20385 (GNG29_20385) mtgA 4273039..4273764 (-) 726 WP_156655277.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GNG29_RS20390 (GNG29_20390) elbB 4273761..4274414 (-) 654 WP_156655278.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54232.08 Da        Isoelectric Point: 4.3230

>NTDB_id=405497 GNG29_RS20360 WP_103177192.1 4269556..4270989(+) (rpoN/rpoN1) [Leclercia sp. Colony189 strain 1106151]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVETQQSQDNETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIELEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIISEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=405497 GNG29_RS20360 WP_103177192.1 4269556..4270989(+) (rpoN/rpoN1) [Leclercia sp. Colony189 strain 1106151]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
ACTGTCGACGCTGGAACTGCAGCAGGAGCTCCAGCAAGCGCTGGAAAGTAACCCTCTGCTCGAGCAAACCGATCTTCACG
ATGAGGTAGAAACGCAACAAAGCCAGGATAACGAGACCCTCGACACGGTGGATGCTCTTGAGCAAAAAGAGATGCCTGAC
GAGCTCCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCAGACTACCAGGATGA
CGAGCTGCCGGTCTATCAGGGCGAGACCACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCGTTCT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTGGATGCCGTGGATGAGACCGGCTATCTGACCGTGTCGCTGGACGAT
ATTCTGGAGAGCATAGGCGATGACGAGATTGAGCTTGAAGAGGTCGAAGCGGTCCTGAAACGCGTTCAGCGCTTTGACCC
GATTGGCGTTGCCGCGAGAGATCTCCGTGACTGCCTGCTCATTCAGCTTTCGCAGTTTGTTAAAGAGACCCCGTGGCTGG
AAGAGGCCCGGCTTATCATCAGCGAACATCTGGATCTGCTTGCCAACCACGATTTCCGCTCACTGATGCGCGTCACGCGG
CTGAAAGAGGAGATCCTGAAAGAGGCGGTCAACCTGATCCAGTCGCTGGATCCGCGCCCGGGGCAATCTATCCAGACCAG
CGAGCCGGAGTATGTTATTCCCGATGTGCTGGTGCGCAAACATAACGGTCTGTGGACCGTAGAGCTGAATTCCGACAGCA
TTCCCCGCCTGCAAATCAATCAGCAATACGCCTCAATGTGCACCAGCTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGTAATCTGCAGGAAGCCCGCTGGCTGATCAAAAGCCTTGAGAGCCGCAATGATACACTACTGCGCGTCAGCCGCTGCAT
CGTGGAGCAGCAACAGGCGTTTTTTGAGCAAGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAGTCGACCATTTCTCGCGTGACGACGCAGAAGTATCTGCACAGTCCGCGTGGCATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCGCTGAGTGACAGTAAGTTGACCACCATGCTGTCCGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.525

100

0.491

Multiple sequence alignment