Detailed information NTG0405453

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GOQ07_RS04705
• Genome accession: NZ_CP046554
• Coordinates: 969219..970337 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 19WIARLN011_full
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 964219..975337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GOQ07_RS04675 (GOQ07_04640)	-	965048..965896 (+)	849	WP_304506014.1	class II glutamine amidotransferase	-
GOQ07_RS04680 (GOQ07_04645)	-	966149..966604 (+)	456	WP_000782976.1	bacteriohemerythrin	-
GOQ07_RS04685 (GOQ07_04650)	-	966757..967824 (+)	1068	WP_029777899.1	membrane protein	-
GOQ07_RS04690 (GOQ07_04655)	-	967824..968147 (+)	324	WP_000401389.1	RnfH family protein	-
GOQ07_RS04695 (GOQ07_04660)	-	968202..968600 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
GOQ07_RS04700 (GOQ07_04665)	fur	968712..969149 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
GOQ07_RS04705 (GOQ07_04670)	pilU	969219..970337 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GOQ07_RS04710 (GOQ07_04675)	pilT	970365..971402 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
GOQ07_RS04715 (GOQ07_04680)	-	971529..972221 (+)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=405453 GOQ07_RS04705 WP_000347034.1 969219..970337(-) (pilU) [Acinetobacter baumannii strain 19WIARLN011_full]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=405453 GOQ07_RS04705 WP_000347034.1 969219..970337(-) (pilU) [Acinetobacter baumannii strain 19WIARLN011_full]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39