Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HXS77_RS18125 Genome accession   NZ_CP058355
Coordinates   3768705..3769145 (+) Length   146 a.a.
NCBI ID   WP_000360896.1    Uniprot ID   -
Organism   Escherichia coli strain AMSCJX03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3763705..3774145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS77_RS18105 (HXS77_18105) aroP 3764703..3766076 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  HXS77_RS18110 (HXS77_18110) ampE 3766119..3766973 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HXS77_RS18115 (HXS77_18115) ampD 3766970..3767521 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HXS77_RS18120 (HXS77_18120) nadC 3767609..3768502 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HXS77_RS18125 (HXS77_18125) pilA 3768705..3769145 (+) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  HXS77_RS18130 (HXS77_18130) pilB 3769155..3770540 (+) 1386 WP_001025156.1 type II secretion system protein GspE Machinery gene
  HXS77_RS18135 (HXS77_18135) hofC 3770530..3771732 (+) 1203 WP_050010183.1 protein transport protein HofC -
  HXS77_RS18140 (HXS77_18140) guaC 3771767..3772810 (-) 1044 WP_001217351.1 GMP reductase -
  HXS77_RS18145 (HXS77_18145) - 3772966..3773010 (-) 45 WP_120795372.1 protein YacM -
  HXS77_RS18150 (HXS77_18150) coaE 3773035..3773655 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15647.84 Da        Isoelectric Point: 4.3938

>NTDB_id=405428 HXS77_RS18125 WP_000360896.1 3768705..3769145(+) (pilA) [Escherichia coli strain AMSCJX03]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQEILNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=405428 HXS77_RS18125 WP_000360896.1 3768705..3769145(+) (pilA) [Escherichia coli strain AMSCJX03]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAATTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment