Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GJR85_RS06195 Genome accession   NZ_CP046528
Coordinates   1268191..1269567 (-) Length   458 a.a.
NCBI ID   WP_156661367.1    Uniprot ID   -
Organism   Lactobacillus acetotolerans strain CN247     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1263191..1274567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJR85_RS06170 (GJR85_06170) - 1263210..1263785 (-) 576 WP_063507492.1 sigma-70 family RNA polymerase sigma factor -
  GJR85_RS06175 (GJR85_06175) rlmB 1263912..1264667 (-) 756 WP_063507493.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GJR85_RS06180 (GJR85_06180) - 1264654..1265097 (-) 444 WP_063507494.1 Mini-ribonuclease 3 -
  GJR85_RS06185 (GJR85_06185) cysS 1265084..1266514 (-) 1431 WP_063507495.1 cysteine--tRNA ligase -
  GJR85_RS06190 (GJR85_06190) gltX 1266614..1268113 (-) 1500 WP_063507496.1 glutamate--tRNA ligase -
  GJR85_RS06195 (GJR85_06195) radA 1268191..1269567 (-) 1377 WP_156661367.1 DNA repair protein RadA Machinery gene
  GJR85_RS06200 (GJR85_06200) - 1269568..1270125 (-) 558 WP_063507985.1 dUTP diphosphatase -
  GJR85_RS06205 (GJR85_06205) - 1270247..1270549 (+) 303 WP_060459231.1 hypothetical protein -
  GJR85_RS06210 (GJR85_06210) pepC 1270630..1271979 (+) 1350 WP_063507984.1 aminopeptidase C -
  GJR85_RS06215 (GJR85_06215) - 1272041..1273435 (-) 1395 WP_238380633.1 IS3 family transposase -
  GJR85_RS06220 (GJR85_06220) - 1273503..1274438 (-) 936 WP_063507766.1 AAA family ATPase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50252.04 Da        Isoelectric Point: 8.7549

>NTDB_id=405129 GJR85_RS06195 WP_156661367.1 1268191..1269567(-) (radA) [Lactobacillus acetotolerans strain CN247]
MARIKTKYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSAKASPSRLIKKTGINEPVKLDKVKAEKDDRIATKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSKKYKVLYVSGEESASQIKLRANRLGLGASDMLLYPETDMHDI
REQINDVQPDFVVIDSIQTMNEPSLDTMTGSASQVREVTSELMKIAKMDAITIFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDKHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDERLPRSTGSSVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMSVASSYTNKEISATDCFVGEVGL
TGEVRRVNKIQARVNEAAKVGFKRIFIPRHNMYKGLKDLGIEVVPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=405129 GJR85_RS06195 WP_156661367.1 1268191..1269567(-) (radA) [Lactobacillus acetotolerans strain CN247]
ATGGCCCGTATAAAAACCAAATATAAGTGCCGTTCTTGTGGTTATATTTCTGCAAGTTATTTAGGGCGCTGTCCTAATTG
TGGCGCCTGGAATCAATTTGAAAAGGAAACGGAAGAAGTTCAAAAGCGTTCTGCCAAGGCCAGCCCCAGTCGGTTGATTA
AGAAAACAGGTATAAATGAGCCTGTTAAGTTAGATAAAGTTAAAGCGGAAAAAGACGACCGGATAGCCACTAAATCTGAA
GAATTGAACCGTGTTTTAGGTGGTGGTATTGTACCCGGCTCACTTGTATTAATCGGTGGGGATCCTGGAATAGGTAAATC
TACTTTGATGTTACAAATCATGAGTGATCTATCTAAAAAATATAAAGTTCTTTATGTTTCTGGAGAGGAATCAGCTAGTC
AGATTAAGCTGCGTGCCAATCGTTTAGGCTTAGGTGCTAGTGATATGCTTTTATATCCAGAAACAGATATGCACGATATT
CGTGAGCAAATTAATGATGTACAGCCTGATTTCGTTGTAATTGATTCTATCCAAACAATGAATGAGCCAAGTTTAGATAC
AATGACTGGCTCAGCTTCACAGGTTCGGGAAGTAACCAGTGAGTTGATGAAAATTGCTAAAATGGACGCAATTACTATTT
TTGTTATTGGCCATGTTACTAAAGAGGGAGCAATTGCTGGGCCAAAAATCATGGAGCATATGGTGGATACCGTTCTTTAC
TTTGAAGGAGATAAACACCACTCCTATCGAATTTTACATTCTGTAAAAAATCGTTTCGGTGCTGCTAATGAAATTGGGAT
GTTTGAGATGGAAAATGAGGGCTTAAAAGAAGTTACTAATCCTTCATCAATCTTTCTTGACGAACGTTTACCGCGTTCGA
CGGGTTCATCCGTTGTGGTTTCACTTGAAGGAACACGGCCTCTGTTAGCTGAAATTCAGGCGCTGGTCACACCAACAGCG
TTTGGTTATGCTAAGAGGACTACATCGGGTATTGATTATAATCGAGCCGCCCTTCTTTTGGCGGTACTTGAAAAAAGAGG
CAACCTTATGCTGCAAAATCAGGATGTATATCTTACCGCGACCGGTGGTATTAAATTAAACGAACCCGCAATCGATCTAG
CTATAACGATGTCTGTGGCATCAAGTTACACTAACAAAGAAATAAGTGCTACAGATTGTTTTGTAGGCGAAGTTGGTTTG
ACTGGAGAAGTTCGGCGGGTAAATAAAATTCAGGCGCGAGTTAATGAGGCAGCTAAGGTTGGGTTTAAACGGATCTTTAT
TCCCCGTCACAATATGTATAAGGGACTAAAAGATTTGGGAATTGAAGTTGTTCCCGTTTCGAGTATCCCGCAAGCTTTAA
AGCTTGTTTTTGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

58.206

99.782

0.581
  radA Streptococcus mitis NCTC 12261

58.206

99.782

0.581
  radA Streptococcus pneumoniae Rx1

58.206

99.782

0.581
  radA Streptococcus pneumoniae D39

58.206

99.782

0.581
  radA Streptococcus pneumoniae R6

58.206

99.782

0.581
  radA Streptococcus pneumoniae TIGR4

58.206

99.782

0.581
  radA Bacillus subtilis subsp. subtilis str. 168

54.902

100

0.55

Multiple sequence alignment