Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GNX71_RS02705 Genome accession   NZ_CP046508
Coordinates   585350..586939 (+) Length   529 a.a.
NCBI ID   WP_206176902.1    Uniprot ID   -
Organism   Variovorax sp. RKNM96     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580350..591939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS02680 (GNX71_02670) argS 580350..582035 (+) 1686 WP_206176897.1 arginine--tRNA ligase -
  GNX71_RS02685 (GNX71_02675) - 582047..582811 (+) 765 WP_206176898.1 SPOR domain-containing protein -
  GNX71_RS02690 (GNX71_02680) - 582978..583625 (+) 648 WP_206176899.1 thiol:disulfide interchange protein DsbA/DsbL -
  GNX71_RS02695 (GNX71_02685) lptA 583824..584540 (+) 717 WP_206176900.1 lipopolysaccharide transport periplasmic protein LptA -
  GNX71_RS02700 (GNX71_02690) lptB 584537..585331 (+) 795 WP_206176901.1 LPS export ABC transporter ATP-binding protein -
  GNX71_RS02705 (GNX71_02695) rpoN/rpoN1 585350..586939 (+) 1590 WP_206176902.1 RNA polymerase factor sigma-54 Machinery gene
  GNX71_RS02710 (GNX71_02700) - 586950..588551 (+) 1602 WP_206176903.1 phospholipase D family protein -
  GNX71_RS02715 (GNX71_02705) - 588562..589206 (-) 645 WP_206176904.1 LysE family translocator -
  GNX71_RS02720 (GNX71_02710) - 589242..589799 (-) 558 WP_206176905.1 DNA-3-methyladenine glycosylase I -
  GNX71_RS02725 (GNX71_02715) - 589831..590466 (-) 636 WP_206176906.1 YceI family protein -
  GNX71_RS02730 (GNX71_02720) - 590517..591086 (-) 570 WP_206176907.1 YceI family protein -
  GNX71_RS02735 (GNX71_02725) - 591100..591693 (-) 594 WP_206176908.1 cytochrome b -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58562.88 Da        Isoelectric Point: 4.6328

>NTDB_id=404767 GNX71_RS02705 WP_206176902.1 585350..586939(+) (rpoN/rpoN1) [Variovorax sp. RKNM96]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPVPRDDGGETGEGD
FAPVPTSSATTATETTSTPDTDGPAAEITEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESLQ
SFLHRQALSLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDLG
ECLGIQLRALASEDETDEEELIRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQIIARLEPKPGRRFVDVERN
IVIPDVIVTKTGRGTNIKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=404767 GNX71_RS02705 WP_206176902.1 585350..586939(+) (rpoN/rpoN1) [Variovorax sp. RKNM96]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCTTTGACGCCACAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTCGAGCAGATGCTGGACGAGAACCCGTTCCTCGAGCGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTCGATGCCGTCGACACGCCGGTCCCGCGCGACGACGGTGGCGAAACCGGTGAAGGCGAT
TTCGCCCCGGTCCCGACCAGCAGTGCGACGACCGCGACCGAAACCACCTCGACACCCGACACCGACGGCCCCGCCGCCGA
GATCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACTGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCGACG
CCCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAATCGCTGCAA
TCGTTCCTGCACCGCCAGGCGCTGAGCCTGCGCCTGAACGAGAACGACTGCGCCGCGCTGCGCTTTTTGATCGAATCGCT
CAACGACGACGGCTACCTCGAAGACTCGCTCCCCGCGCTGGCCTCGGGCCTGGCGGGCGACGACAACGACCAGTTCGACG
AGCTCGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCCGCCGGCGTCGGCGCGCGCGACCTGGGC
GAGTGCCTGGGCATCCAGTTGCGCGCGCTCGCCAGCGAAGACGAGACCGACGAAGAAGAGCTCATCCGCAAGACCGCCAT
CGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGGACCAACGAAG
AGCTGGTGCGCTCGGCGCTGCAGATCATTGCGCGCCTGGAGCCCAAGCCGGGGCGCCGTTTCGTCGACGTCGAGCGCAAC
ATCGTCATTCCCGATGTGATCGTCACCAAGACCGGCCGCGGCACCAACATCAAGTTCCGCGTCATGCTCAACCCTGAGGT
GATGCCGCGCCTGCGCGTGCACGACATCTACGCGGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGTTGGTTCATCAAGAACATCCAGCAGCGTTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTGGAGCGGCAGAAGAGCTACTTCGTGCACGGCGAACTCGCGATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCCACGCCGTTCGGCACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTCATCAAGCAGTTC
GTGAGTTCCGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCCGCGAATCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.128

100

0.514

Multiple sequence alignment