Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GNX71_RS01420 Genome accession   NZ_CP046508
Coordinates   305841..307229 (+) Length   462 a.a.
NCBI ID   WP_093440937.1    Uniprot ID   A0A1I5WIA3
Organism   Variovorax sp. RKNM96     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 300841..312229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS01390 (GNX71_01380) - 301120..301455 (-) 336 WP_206176669.1 aromatic ring-opening dioxygenase subunit LigA -
  GNX71_RS01395 (GNX71_01385) - 301459..302433 (-) 975 WP_206176670.1 tripartite tricarboxylate transporter substrate binding protein -
  GNX71_RS01400 (GNX71_01390) hpaE 302430..303893 (-) 1464 WP_206176671.1 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase -
  GNX71_RS01405 (GNX71_01395) - 303890..304675 (-) 786 WP_206176672.1 fumarylacetoacetate hydrolase family protein -
  GNX71_RS01410 (GNX71_01400) - 304672..305310 (-) 639 WP_206176673.1 fumarylacetoacetate hydrolase family protein -
  GNX71_RS01415 (GNX71_01405) hpaR 305307..305741 (-) 435 WP_013538719.1 homoprotocatechuate degradation operon regulator HpaR -
  GNX71_RS01420 (GNX71_01410) radA 305841..307229 (+) 1389 WP_093440937.1 DNA repair protein RadA Machinery gene
  GNX71_RS01425 (GNX71_01415) - 307779..308207 (+) 429 WP_206176674.1 glycerate kinase -
  GNX71_RS01430 (GNX71_01420) - 308204..309148 (-) 945 WP_206176675.1 LysR substrate-binding domain-containing protein -
  GNX71_RS01435 (GNX71_01425) - 309279..310637 (+) 1359 WP_206176676.1 MFS transporter -
  GNX71_RS01440 (GNX71_01430) - 310680..311891 (+) 1212 WP_206176677.1 M20 aminoacylase family protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 48625.90 Da        Isoelectric Point: 6.9555

>NTDB_id=404765 GNX71_RS01420 WP_093440937.1 305841..307229(+) (radA) [Variovorax sp. RKNM96]
MAKDKSLFVCSECGGTSPKWLGKCPSCGAWNTLIEQVAGSNAPANNRFGTQFSPLAGVSELATLSEIEATDIARTPTGID
ELDRVLGGGIVSGGVTLIGGDPGIGKSTLLLQAVDALQRAGQNALYVTGEESGAQVALRSRRLGLDHSQVQVLAEIQLEK
IIATLDATRPAIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRFAKTSGTAVVLVGHVTKEGALAGPRVLEHMVDTVL
YFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTERGLKGVANPSAIFLSQHAEPVPGSVVLVTLEGTRPMLVEIQALVDNG
GPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKPLPKGFIAFGEVGL
AGEVRPAPRGQERLREAAKLGFSIAVVPKANAPRKGSKEIEGLTIHPVERIEEAMDVVRRLD

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=404765 GNX71_RS01420 WP_093440937.1 305841..307229(+) (radA) [Variovorax sp. RKNM96]
ATGGCCAAGGACAAATCTCTTTTCGTCTGCAGCGAATGCGGCGGCACCAGCCCCAAGTGGCTCGGCAAATGCCCGAGCTG
CGGCGCCTGGAACACATTGATCGAACAGGTGGCGGGCAGCAACGCACCAGCCAACAACCGCTTCGGCACGCAGTTCTCGC
CGCTGGCGGGTGTGTCCGAACTGGCCACGCTCTCGGAGATCGAGGCGACCGACATCGCGCGCACGCCCACCGGCATCGAC
GAACTCGACCGCGTGCTGGGCGGCGGCATCGTCTCGGGCGGCGTCACGCTGATCGGCGGCGACCCGGGCATCGGCAAGTC
GACGCTGCTGCTGCAGGCGGTCGATGCGCTGCAGCGCGCGGGGCAGAACGCGCTCTACGTGACGGGCGAGGAAAGCGGCG
CGCAGGTGGCGCTGCGCTCGCGCCGGCTCGGACTCGATCACTCGCAGGTGCAGGTGCTCGCCGAGATCCAGCTCGAGAAG
ATCATCGCCACGCTGGACGCCACGCGGCCCGCGATCGCGGTGATCGACTCCATTCAAACGGTGTATTCAGACCAGCTCAC
CTCGGCCCCCGGCTCGGTGGCGCAGGTGCGCGAATGCGCGGCCCATCTCACGCGCTTTGCCAAGACCAGCGGCACGGCCG
TGGTGCTGGTGGGCCATGTCACGAAAGAGGGCGCGCTCGCAGGTCCGCGTGTGCTCGAGCACATGGTCGACACGGTGCTG
TATTTCGAGGGTGACACGCATTCGAGCTTCCGCCTCGTGCGTGCCATCAAGAACCGCTTCGGGGCCGTGAACGAGATCGG
CGTGTTCGCGATGACCGAGCGCGGCTTGAAGGGCGTGGCCAATCCGAGCGCGATCTTCCTGAGCCAGCACGCCGAGCCGG
TGCCCGGCAGCGTGGTGCTGGTCACGCTCGAGGGCACGCGGCCGATGCTGGTGGAAATCCAGGCGCTGGTCGACAACGGC
GGGCCGAGCCCGCGGCGCCTCTCGGTGGGCCTGGACCGCGACCGCCTCGCGATGCTGCTCGCAGTGCTGCACCGCCATGC
GGGCGTGGCCTGCATGGACCAGGACGTGTTCGTGAATGCGGTGGGCGGCGTGCGCATCAGCGAACCCGCGGCCGACCTGG
CCGTGATGCTGGCCATCACCTCGAGCCTGCGCGGCAAGCCGCTGCCCAAAGGGTTCATCGCGTTCGGTGAAGTGGGCCTG
GCCGGTGAAGTGCGCCCCGCGCCGCGCGGGCAGGAGCGTTTGCGCGAAGCCGCCAAGCTGGGCTTCAGCATCGCGGTGGT
GCCCAAGGCCAATGCGCCGCGCAAGGGCTCGAAAGAGATCGAGGGGCTGACCATCCACCCGGTCGAGCGCATCGAAGAGG
CGATGGACGTGGTCCGCCGCCTCGATTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(362-456)

AAA domain (AAA_25)

(65-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I5WIA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

98.701

0.481
  radA Streptococcus mitis SK321

47.806

93.723

0.448
  radA Streptococcus pneumoniae Rx1

47.806

93.723

0.448
  radA Streptococcus pneumoniae D39

47.806

93.723

0.448
  radA Streptococcus pneumoniae R6

47.806

93.723

0.448
  radA Streptococcus pneumoniae TIGR4

47.806

93.723

0.448
  radA Streptococcus mitis NCTC 12261

47.575

93.723

0.446

Multiple sequence alignment