Detailed information NTG0404615

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HUU85_RS09935
• Genome accession: NZ_CP058302
• Coordinates: 2065239..2066222 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain AH65
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 2060239..2071222

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUU85_RS09910 (HUU85_09920)	bcsF	2060400..2060591 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
HUU85_RS09915 (HUU85_09925)	bcsG	2060588..2062267 (+)	1680	WP_176256414.1	cellulose biosynthesis protein BcsG	-
HUU85_RS09920 (HUU85_09930)	ldrD	2062354..2062461 (-)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
HUU85_RS09925 (HUU85_09935)	yhjV	2062937..2064208 (+)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
HUU85_RS09930 (HUU85_09940)	dppF	2064238..2065242 (-)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HUU85_RS09935 (HUU85_09945)	amiE	2065239..2066222 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
HUU85_RS09940 (HUU85_09950)	dppC	2066233..2067135 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HUU85_RS09945 (HUU85_09955)	dppB	2067145..2068164 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
HUU85_RS24855	-	2068328..2068438 (+)	111	Protein_1962	hypothetical protein	-
HUU85_RS09950 (HUU85_09960)	dppA	2068472..2070079 (-)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=404615 HUU85_RS09935 WP_001196486.1 2065239..2066222(-) (amiE) [Escherichia coli strain AH65]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=404615 HUU85_RS09935 WP_001196486.1 2065239..2066222(-) (amiE) [Escherichia coli strain AH65]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404