Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   HUU85_RS02420 Genome accession   NZ_CP058302
Coordinates   467116..468543 (+) Length   475 a.a.
NCBI ID   WP_000980610.1    Uniprot ID   -
Organism   Escherichia coli strain AH65     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 462116..473543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU85_RS02380 (HUU85_02390) yeeW 462319..462513 (+) 195 WP_000988599.1 DUF5983 family protein -
  HUU85_RS02385 (HUU85_02395) - 462559..462639 (+) 81 Protein_473 hypothetical protein -
  HUU85_RS02390 (HUU85_02400) - 462928..463074 (-) 147 Protein_474 transposase domain-containing protein -
  HUU85_RS02395 (HUU85_02405) - 463128..463310 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  HUU85_RS02400 (HUU85_02410) tmaR 463411..463740 (-) 330 WP_000450409.1 DUF496 family protein -
  HUU85_RS02405 (HUU85_02415) yeeA 463912..464970 (-) 1059 WP_001200891.1 FUSC family protein -
  HUU85_RS02410 (HUU85_02420) sbmC 465169..465642 (-) 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
  HUU85_RS02415 (HUU85_02425) dacD 465761..466927 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  HUU85_RS02420 (HUU85_02430) sbcB 467116..468543 (+) 1428 WP_000980610.1 exodeoxyribonuclease I Machinery gene
  HUU85_RS02425 (HUU85_02435) tsuB 468586..468813 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  HUU85_RS02430 (HUU85_02440) tsuA 468827..469885 (-) 1059 WP_176256508.1 thiosulfate utilization transporter TsuA/YeeE -
  HUU85_RS02435 (HUU85_02445) plaP 470064..471422 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  HUU85_RS02440 (HUU85_02450) yoeI 471412..471474 (-) 63 WP_010723108.1 membrane protein YoeI -
  HUU85_RS02445 (HUU85_02455) yeeY 471689..472618 (-) 930 WP_000803360.1 LysR substrate-binding domain-containing protein -
  HUU85_RS02450 (HUU85_02460) yeeZ 472664..473488 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54472.76 Da        Isoelectric Point: 5.4720

>NTDB_id=404586 HUU85_RS02420 WP_000980610.1 467116..468543(+) (sbcB) [Escherichia coli strain AH65]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=404586 HUU85_RS02420 WP_000980610.1 467116..468543(+) (sbcB) [Escherichia coli strain AH65]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTGCCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTGCTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCAGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGCGCAGCAATGAAAATTGTGCTGGAAACCGAGCCACGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment