Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   HWU09_RS07160 Genome accession   NZ_CP058287
Coordinates   1492714..1495083 (+) Length   789 a.a.
NCBI ID   WP_237003353.1    Uniprot ID   -
Organism   Helicobacter pylori strain AL04     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1487714..1500083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWU09_RS07130 - 1488450..1489304 (-) 855 WP_237003350.1 NAD(+)/NADH kinase -
  HWU09_RS07135 - 1489479..1490258 (+) 780 WP_237003351.1 hypothetical protein -
  HWU09_RS07140 - 1490278..1490571 (+) 294 WP_154490397.1 hypothetical protein -
  HWU09_RS07145 - 1490578..1492101 (+) 1524 WP_237003352.1 toprim domain-containing protein -
  HWU09_RS07150 - 1492098..1492400 (+) 303 WP_031247542.1 hypothetical protein -
  HWU09_RS07155 comB3 1492401..1492667 (+) 267 WP_001173705.1 hypothetical protein Machinery gene
  HWU09_RS07160 comB4 1492714..1495083 (+) 2370 WP_237003353.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  HWU09_RS07165 - 1495086..1497119 (+) 2034 WP_237003354.1 type IA DNA topoisomerase -
  HWU09_RS07170 - 1497236..1498405 (+) 1170 WP_237003355.1 type IV secretion system protein -
  HWU09_RS07175 - 1498405..1499949 (+) 1545 WP_237003356.1 TrbG/VirB9 family P-type conjugative transfer protein -

Sequence

Protein


Download         Length: 789 a.a.        Molecular weight: 91285.78 Da        Isoelectric Point: 8.1748

>NTDB_id=404399 HWU09_RS07160 WP_237003353.1 1492714..1495083(+) (comB4) [Helicobacter pylori strain AL04]
MKEETNIVGIYNEHYLLSEKSNLVGALKLEGISYLSLDDKEIAQKFNERILALNEIVDGVHFKIVAKRRKIFMHHEYTQE
EISNPFALEVINLWEQGVEDVYQNFYYLIFETKNDSIKGYLERFKKKITTNELENIQENNKQDEVKYQENYFIGFDNFNL
LDKSKILDSIINNIKNMLSGLFVEKIDANSLLNFYAEYINGVPSEYTFARGRLHDGMINSDVHFKKDFFTHIHNNKEIFK
RFISIKTYDIDKIVSTALSSVLHLSLEFDVILNIDNIPKAKAEKRIETKRKRANKSIRVEIDKLNDMIKTDRVLMQEISL
NILVHAKTKTDLDSACIEITNLLKQKGIVSTQESIGMLPMFFSFFPNRNRLNFRKRLLSSQNIASLIILEKQNCGFSRNS
WGDRHLTIFRNQDKSPYLFNFHAYEAKRKNDMVSGHTIIFGGTGAGKTTLIEFLITNCFKYEDLSILALDRNNGMRVMTE
FLDGQYNDSNDFYINPFSLKDTSANCTFLVSWLAFMLNIDEDTKDEKEKKTLSALSKTIRVCYNNITKQGGAIFKLRDFK
DTLERDYLDSKDILEKESSKDLYKHSTDCLDFTKKLSVIDMDALSSSKKDFNLAVLYLFYKVMNRAKLENKPFYIFIDEA
KSVIENPIMLVKIKDTLAQARKLNGVLTLAFQDINQLDGVEGAKSIIENAAQVILYPTNNFEKLESYGILLSPIEKSFLN
KTPLNARQVLVKNLLTQSSVFLEINLEKLNSTSKKFLRAYSSSASSVFELETLKKDNPKNYKEIYLTKE

Nucleotide


Download         Length: 2370 bp        

>NTDB_id=404399 HWU09_RS07160 WP_237003353.1 1492714..1495083(+) (comB4) [Helicobacter pylori strain AL04]
ATGAAAGAAGAAACTAACATAGTGGGCATTTACAATGAGCATTATCTTTTAAGTGAAAAATCAAACCTTGTAGGCGCTCT
TAAGTTAGAGGGAATTTCTTATTTGTCTTTAGATGACAAAGAAATAGCGCAAAAATTTAATGAACGCATTTTAGCTCTTA
ATGAGATTGTAGATGGTGTGCATTTTAAAATCGTAGCTAAAAGGCGTAAGATTTTCATGCACCATGAATATACTCAAGAG
GAGATAAGCAATCCTTTTGCATTAGAGGTTATCAATTTATGGGAGCAGGGGGTTGAGGATGTTTATCAAAATTTCTATTA
CTTAATTTTTGAAACTAAGAATGATAGCATTAAAGGTTATTTGGAACGATTTAAGAAAAAAATCACTACCAATGAATTAG
AAAATATTCAAGAAAATAACAAACAAGATGAAGTTAAATATCAAGAAAACTATTTCATAGGCTTTGATAATTTTAATCTA
TTAGATAAATCTAAAATATTAGACAGCATTATCAATAATATTAAAAACATGCTTTCTGGCTTGTTTGTAGAAAAAATAGA
TGCCAACAGTTTGCTTAATTTTTATGCGGAATATATCAATGGCGTTCCTAGTGAATACACTTTTGCTAGAGGTCGTTTGC
ATGATGGCATGATTAATTCTGATGTGCATTTTAAAAAAGATTTTTTCACTCACATTCATAATAATAAAGAGATTTTCAAG
CGTTTCATCAGCATTAAGACTTATGATATAGACAAAATTGTCAGCACCGCTCTTTCTTCAGTATTGCATTTATCTTTAGA
ATTCGATGTGATTTTAAACATTGACAATATTCCTAAAGCAAAGGCTGAAAAAAGGATTGAAACTAAAAGAAAAAGAGCGA
ATAAGAGCATTAGAGTTGAAATTGATAAATTGAATGACATGATTAAAACAGACAGGGTTTTGATGCAGGAGATTTCTTTA
AACATTTTAGTTCATGCCAAAACTAAAACCGATTTAGATAGTGCTTGTATAGAAATTACCAACTTGCTTAAACAAAAAGG
TATTGTAAGCACACAAGAGAGTATTGGCATGTTGCCTATGTTTTTTAGCTTTTTCCCCAATCGTAATCGCTTGAATTTTA
GAAAGCGTTTGTTGTCTAGTCAAAATATTGCTTCTTTAATCATATTGGAAAAACAAAATTGTGGTTTTTCTAGAAATTCA
TGGGGTGATCGCCATTTAACTATTTTTAGAAACCAAGACAAATCGCCTTATTTATTCAATTTCCATGCTTATGAAGCAAA
AAGAAAAAATGATATGGTATCAGGGCATACCATCATCTTTGGTGGCACAGGGGCTGGTAAAACCACTTTAATTGAATTTT
TAATCACCAATTGTTTTAAATATGAAGATTTAAGTATTTTGGCTTTAGACAGAAATAATGGCATGCGTGTGATGACTGAG
TTTTTAGATGGTCAATACAACGATAGCAATGATTTCTACATTAATCCGTTTTCTTTAAAAGATACAAGCGCTAATTGCAC
CTTTTTAGTGAGTTGGTTGGCATTCATGCTCAATATTGATGAAGATACTAAAGATGAAAAAGAAAAAAAGACTTTAAGCG
CTTTATCCAAAACGATCAGAGTTTGCTATAACAATATTACAAAACAAGGGGGTGCTATTTTTAAGTTAAGAGATTTTAAA
GACACGCTAGAAAGAGACTATTTAGATAGTAAAGACATTTTAGAAAAAGAGAGCTCAAAAGACTTATACAAGCATTCTAC
TGATTGTTTAGACTTTACTAAAAAGCTTTCTGTTATTGATATGGATGCTCTTTCTAGTAGTAAAAAAGATTTCAACTTGG
CAGTGCTATACCTATTCTACAAAGTCATGAATAGAGCTAAATTAGAAAACAAGCCTTTTTACATATTCATTGATGAAGCA
AAATCTGTTATTGAAAATCCCATCATGTTAGTCAAAATCAAAGACACACTCGCTCAAGCTAGAAAACTCAATGGGGTTTT
AACTTTAGCCTTTCAAGATATTAACCAACTTGATGGTGTAGAGGGAGCAAAGTCCATTATAGAAAACGCCGCTCAAGTCA
TACTCTATCCTACAAACAATTTTGAGAAATTAGAGAGCTATGGCATTTTATTAAGTCCTATTGAAAAATCATTTTTAAAC
AAAACGCCGTTAAACGCTAGGCAAGTATTAGTGAAAAACCTTCTCACTCAATCTAGCGTATTTTTAGAAATCAATTTAGA
AAAGCTAAACAGCACCAGTAAAAAATTTTTAAGAGCGTATAGCTCTAGTGCGTCTAGCGTTTTTGAACTGGAAACGCTTA
AAAAGGATAATCCAAAGAATTATAAAGAAATTTACTTGACAAAGGAGTAG

Domains

Predicted by InterProScan.

Helicase HerA, central domain (DUF87)

(430-472)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-374)

TraM recognition site of TraD and TraG (TraG-D_C)

(632-706)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

35.975

100

0.362

Multiple sequence alignment