Detailed information NTG0404368

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GFX43_RS02820
• Genome accession: NZ_CP046448
• Coordinates: 535919..537064 (-)
• Length: 381 a.a.
• NCBI ID: WP_015384935.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain ZD01
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 530919..542064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFX43_RS02795 (GFX43_002795)	-	531487..532050 (-)	564	WP_015483840.1	hypothetical protein	-
GFX43_RS02800 (GFX43_002800)	-	532043..532690 (-)	648	WP_015384940.1	ABC transporter ATP-binding protein	-
GFX43_RS02805 (GFX43_002805)	-	534166..534852 (-)	687	WP_236822444.1	hypothetical protein	-
GFX43_RS02810 (GFX43_002810)	-	535112..535588 (-)	477	WP_015384936.1	hypothetical protein	-
GFX43_RS02815 (GFX43_002815)	phrF	535816..535935 (-)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
GFX43_RS02820 (GFX43_002820)	rapF	535919..537064 (-)	1146	WP_015384935.1	response regulator aspartate phosphatase RapF	Regulator
GFX43_RS20885 (GFX43_002825)	-	537067..537297 (+)	231	WP_015483838.1	hypothetical protein	-
GFX43_RS02830 (GFX43_002830)	ywhK	537294..538649 (+)	1356	WP_015384933.1	YncE family protein	-
GFX43_RS02835 (GFX43_002835)	ywhL	538689..540062 (+)	1374	WP_015483837.1	YncE family protein	-
GFX43_RS02840 (GFX43_002840)	albG	540068..540769 (-)	702	WP_015384931.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
GFX43_RS02845 (GFX43_002845)	albF	540766..542046 (-)	1281	WP_015483836.1	pitrilysin family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45617.08 Da
• Isoelectric Point: 5.1503

>NTDB_id=404368 GFX43_RS02820 WP_015384935.1 535919..537064(-) (rapF) [Bacillus subtilis strain ZD01]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDHMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=404368 GFX43_RS02820 WP_015384935.1 535919..537064(-) (rapF) [Bacillus subtilis strain ZD01]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTTAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCACATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTAATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAGAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.95	100	0.99
rapC	Bacillus subtilis subsp. subtilis str. 168	57.632	99.738	0.575