Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFX43_RS00360 Genome accession   NZ_CP046448
Coordinates   53332..54708 (-) Length   458 a.a.
NCBI ID   WP_014475593.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZD01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48332..59708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFX43_RS00340 (GFX43_000340) ispF 49848..50324 (-) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GFX43_RS00345 (GFX43_000345) ispD 50317..51015 (-) 699 WP_015382584.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GFX43_RS00350 (GFX43_000350) yacL 51030..52130 (-) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  GFX43_RS00355 (GFX43_000355) disA 52246..53328 (-) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  GFX43_RS00360 (GFX43_000360) radA 53332..54708 (-) 1377 WP_014475593.1 DNA repair protein RadA Machinery gene
  GFX43_RS00365 (GFX43_000365) clpC 54800..57232 (-) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GFX43_RS00370 (GFX43_000370) mcsB 57229..58320 (-) 1092 WP_003235007.1 protein arginine kinase -
  GFX43_RS00375 (GFX43_000375) mcsA 58320..58877 (-) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  GFX43_RS00380 (GFX43_000380) ctsR 58891..59355 (-) 465 WP_003225724.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49466.99 Da        Isoelectric Point: 8.2399

>NTDB_id=404359 GFX43_RS00360 WP_014475593.1 53332..54708(-) (radA) [Bacillus subtilis strain ZD01]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=404359 GFX43_RS00360 WP_014475593.1 53332..54708(-) (radA) [Bacillus subtilis strain ZD01]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACACAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATCGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTGCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGGCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAGACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAAGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAGGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
TGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTTGTTGAAATTCAGGCGCTTATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.782

100

0.998
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

62.914

98.908

0.622

Multiple sequence alignment