Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GNG27_RS20705 Genome accession   NZ_CP046445
Coordinates   4311844..4313277 (+) Length   477 a.a.
NCBI ID   WP_156264726.1    Uniprot ID   -
Organism   Leclercia sp. 119287     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4306844..4318277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNG27_RS20675 (GNG27_20675) - 4307411..4308388 (+) 978 WP_156264724.1 calcium/sodium antiporter -
  GNG27_RS20680 (GNG27_20680) kdsD 4308402..4309388 (+) 987 WP_156264725.1 arabinose-5-phosphate isomerase KdsD -
  GNG27_RS20685 (GNG27_20685) kdsC 4309403..4309969 (+) 567 WP_106994894.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GNG27_RS20690 (GNG27_20690) lptC 4309966..4310541 (+) 576 WP_142487254.1 LPS export ABC transporter periplasmic protein LptC -
  GNG27_RS20695 (GNG27_20695) lptA 4310510..4311064 (+) 555 WP_142487255.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  GNG27_RS20700 (GNG27_20700) lptB 4311071..4311796 (+) 726 WP_142487256.1 LPS export ABC transporter ATP-binding protein -
  GNG27_RS20705 (GNG27_20705) rpoN/rpoN1 4311844..4313277 (+) 1434 WP_156264726.1 RNA polymerase factor sigma-54 Machinery gene
  GNG27_RS20710 (GNG27_20710) hpf 4313300..4313587 (+) 288 WP_142487258.1 ribosome hibernation promoting factor -
  GNG27_RS20715 (GNG27_20715) ptsN 4313670..4314161 (+) 492 WP_039031625.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GNG27_RS20720 (GNG27_20720) rapZ 4314207..4315061 (+) 855 WP_142487259.1 RNase adapter RapZ -
  GNG27_RS20725 (GNG27_20725) npr 4315058..4315330 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  GNG27_RS20730 (GNG27_20730) mtgA 4315327..4316052 (-) 726 WP_370462883.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GNG27_RS20735 (GNG27_20735) elbB 4316049..4316702 (-) 654 WP_156264727.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54175.97 Da        Isoelectric Point: 4.3155

>NTDB_id=404330 GNG27_RS20705 WP_156264726.1 4311844..4313277(+) (rpoN/rpoN1) [Leclercia sp. 119287]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIDLEEIEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=404330 GNG27_RS20705 WP_156264726.1 4311844..4313277(+) (rpoN/rpoN1) [Leclercia sp. 119287]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
GCTGTCAACGCTGGAACTTCAGCAGGAACTCCAGCAGGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ATGAGGTAGAAACTCAGCAAACCCAGGATAACGAAACTCTCGACAGCGTTGATGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGCACGCGCGCAGACTACCAGGATGA
TGAATTGCCGGTGTATCAGGGCGAGACTACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCCTTTT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTGACCGTGTCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTCGAAGAGATCGAAGCGGTTCTGAAGCGCGTTCAGCGTTTTGATCC
GGTCGGCGTCGCGGCACGCGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTTGTTAAAGAGACGCCGTGGCTGG
AAGAGGCTCGCCTGATCGTCAGCGAGCATCTGGATCTGCTGGCTAACCACGACTTCCGCTCCCTGATGCGCGTGACGCGG
CTGAAGGAAGAGATCCTGAAAGAAGCGGTCAATTTGATTCAGTCGCTGGATCCGCGTCCGGGGCAGTCTATCCAGACCAG
CGAGCCCGAGTACGTCATTCCCGACGTGCTGGTGCGCAAGCATAACGGCCTGTGGACCGTGGAATTGAATTCCGACAGCA
TCCCTCGTCTGCAGATAAATCAGCAGTACGCCTCGATGTGCACCAGCGCGCGCAACGACTCGGATAACCAATATATCCGC
AGTAATCTTCAGGAAGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTGCGCGTCAGCCGCTGCAT
CGTGGAACAGCAGCAGGCGTTCTTTGAACAAGGTGAAGAATTTATGAAGCCGATGGTGCTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATTTCCCGCGTGACGACGCAGAAGTATCTGCATAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCTTCGTCGACGGCCATCCGGGCGCTGGTGAAAAAGTT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTTG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment