Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GM418_RS12255 Genome accession   NZ_CP046401
Coordinates   3089864..3091222 (-) Length   452 a.a.
NCBI ID   WP_158866532.1    Uniprot ID   A0A6I6JPL9
Organism   Maribellus comscasis strain WC007     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3084864..3096222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM418_RS12235 (GM418_12230) - 3086866..3087309 (+) 444 WP_158866520.1 DUF2147 domain-containing protein -
  GM418_RS12240 (GM418_12235) - 3087342..3088121 (-) 780 WP_158866523.1 radical SAM protein -
  GM418_RS12245 (GM418_12240) - 3088212..3089405 (-) 1194 WP_158866526.1 amidohydrolase family protein -
  GM418_RS12250 (GM418_12245) - 3089412..3089798 (-) 387 WP_158866529.1 DUF4296 domain-containing protein -
  GM418_RS12255 (GM418_12250) radA 3089864..3091222 (-) 1359 WP_158866532.1 DNA repair protein RadA Machinery gene
  GM418_RS12260 (GM418_12255) - 3091289..3091780 (-) 492 WP_158866535.1 adenylyltransferase/cytidyltransferase family protein -
  GM418_RS12265 (GM418_12260) - 3092281..3093033 (+) 753 WP_158866538.1 hypothetical protein -
  GM418_RS12270 (GM418_12265) - 3093176..3093475 (+) 300 WP_158866541.1 hypothetical protein -
  GM418_RS12275 (GM418_12270) panD 3093531..3093881 (-) 351 WP_158866543.1 aspartate 1-decarboxylase -
  GM418_RS12280 (GM418_12275) panC 3093894..3094733 (-) 840 WP_158866546.1 pantoate--beta-alanine ligase -
  GM418_RS12285 (GM418_12280) - 3094897..3095697 (+) 801 WP_158866548.1 glycogen/starch synthase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49113.66 Da        Isoelectric Point: 8.2895

>NTDB_id=403947 GM418_RS12255 WP_158866532.1 3089864..3091222(-) (radA) [Maribellus comscasis strain WC007]
MPATKSIYTCQNCGFQSPKWMGKCTSCGEWNTFVEEIIEKKKSAAKVAARHLGNQPITLENIQAAKTQRIVAGIKEFDRV
LGGGIVPGSLILLGGDPGIGKSTLALQLALKLNKQKILYTSGEESLQQIKLRAERLNNSNNNCLFLSETSLENLLAHSEQ
IQPDVLIIDSIQTISTENIESSPGSVSQVRECTSAILKFAKKSGVAVVLIGHINKEGSIAGPKVLEHIVDTVLQFEGDTN
YMYRILRSNKNRFGSTNELGIFEMRSDGLREITNPSEQLISKVSDDVSGTAIAATIEGIRPILIEIQALVSSAAYGTPQR
SSTGFDLRRLNMLLAVLEKRAGFKLIQKDVFLNIAGGLKINDPATDLAVICSILSSNIDIPIDHKICFAGEVGLTGEIRA
VNRIEQRIAEAAKLGFKKIIIPSLNKGFDTSGFGIDIVKTSRVEEVFRSIFG

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=403947 GM418_RS12255 WP_158866532.1 3089864..3091222(-) (radA) [Maribellus comscasis strain WC007]
ATGCCGGCAACAAAATCAATATACACGTGCCAGAACTGTGGTTTTCAATCTCCAAAATGGATGGGGAAATGTACCTCTTG
TGGCGAGTGGAATACTTTTGTGGAAGAGATAATCGAAAAAAAGAAAAGTGCCGCAAAAGTAGCCGCCCGGCATTTGGGAA
ACCAGCCGATAACACTGGAAAACATTCAGGCGGCAAAAACGCAACGGATTGTTGCCGGAATTAAGGAATTCGACCGTGTA
CTTGGTGGCGGAATAGTTCCCGGCTCGCTGATTCTTTTGGGCGGTGATCCGGGAATCGGAAAATCGACACTGGCCCTGCA
GCTGGCACTCAAATTAAACAAACAAAAAATTTTATATACATCGGGAGAAGAGAGTCTGCAACAAATAAAACTTCGTGCCG
AGCGACTAAACAATAGCAACAACAATTGTCTTTTTCTGAGTGAAACTTCACTGGAAAATCTGCTCGCACATTCCGAACAA
ATTCAACCCGATGTTTTAATTATCGATTCCATTCAAACCATTTCCACAGAAAATATTGAATCTTCACCCGGGTCAGTTTC
ACAGGTTCGTGAGTGTACGTCGGCCATTTTGAAATTTGCAAAAAAAAGCGGAGTTGCAGTTGTTCTTATTGGTCATATCA
ATAAGGAAGGAAGTATTGCCGGCCCCAAAGTGCTGGAGCATATTGTAGATACGGTTTTGCAGTTTGAAGGTGATACCAAT
TACATGTACCGGATTTTACGTTCGAACAAAAATCGCTTTGGCTCCACCAACGAACTGGGTATTTTTGAAATGCGCAGTGA
CGGATTACGGGAAATTACCAATCCTTCAGAGCAACTGATTTCAAAAGTAAGCGACGATGTAAGTGGCACAGCCATTGCCG
CCACGATTGAAGGAATTCGCCCTATTTTAATCGAAATTCAGGCGCTTGTAAGTTCAGCTGCCTACGGAACACCACAGCGC
TCATCCACCGGATTTGATTTACGCCGGCTAAATATGTTGCTGGCGGTTCTGGAAAAACGAGCCGGTTTTAAACTCATTCA
AAAAGATGTGTTTCTGAATATTGCCGGCGGATTAAAAATAAACGATCCGGCTACCGATTTGGCTGTCATCTGCTCTATTC
TCTCCTCAAATATCGACATTCCCATCGACCATAAAATTTGTTTTGCGGGCGAGGTGGGATTAACAGGCGAAATTCGTGCT
GTAAACCGAATTGAACAACGAATAGCCGAAGCCGCCAAACTGGGATTCAAAAAAATCATTATCCCCTCACTAAACAAAGG
ATTTGATACATCCGGATTTGGAATTGATATTGTAAAAACCAGCCGTGTTGAAGAAGTCTTCAGAAGTATTTTCGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(63-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(340-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6JPL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.985

100

0.515
  radA Streptococcus mitis SK321

49.558

100

0.496
  radA Streptococcus pneumoniae Rx1

49.558

100

0.496
  radA Streptococcus pneumoniae D39

49.558

100

0.496
  radA Streptococcus pneumoniae R6

49.558

100

0.496
  radA Streptococcus pneumoniae TIGR4

49.558

100

0.496
  radA Streptococcus mitis NCTC 12261

49.336

100

0.493

Multiple sequence alignment