Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MDS_RS04445 Genome accession   NC_015410
Coordinates   975601..976821 (-) Length   406 a.a.
NCBI ID   WP_013714029.1    Uniprot ID   A0A379IZL3
Organism   Ectopseudomonas mendocina NK-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970601..981821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDS_RS04405 (MDS_0878) - 970891..971064 (-) 174 WP_013714021.1 DUF3094 family protein -
  MDS_RS04410 (MDS_0879) - 971272..971826 (-) 555 WP_013714022.1 MOSC domain-containing protein -
  MDS_RS04415 (MDS_0880) - 971823..972452 (-) 630 WP_013714023.1 DUF1780 domain-containing protein -
  MDS_RS04420 (MDS_0881) - 972661..973350 (+) 690 WP_013714024.1 energy-coupling factor ABC transporter permease -
  MDS_RS04425 (MDS_0882) - 973456..973671 (+) 216 WP_013714025.1 hypothetical protein -
  MDS_RS04430 (MDS_0883) yacG 973743..973937 (-) 195 WP_013714026.1 DNA gyrase inhibitor YacG -
  MDS_RS04435 (MDS_0884) coaE 973934..974542 (-) 609 WP_013714027.1 dephospho-CoA kinase -
  MDS_RS04440 (MDS_0885) pilD 974731..975597 (-) 867 WP_085982453.1 A24 family peptidase Machinery gene
  MDS_RS04445 (MDS_0886) pilC 975601..976821 (-) 1221 WP_013714029.1 type II secretion system F family protein Machinery gene
  MDS_RS04450 (MDS_0887) pilB 976825..978525 (-) 1701 WP_041769664.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MDS_RS26250 (MDS_0888) - 978753..978938 (+) 186 WP_003242736.1 type IV pilin protein -
  MDS_RS25670 - 978950..979408 (+) 459 WP_147285547.1 hypothetical protein -
  MDS_RS26255 pilA 979497..979676 (+) 180 WP_041769344.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MDS_RS04465 (MDS_0889) - 979849..980553 (+) 705 WP_115299238.1 hypothetical protein -
  MDS_RS04470 (MDS_0890) - 980556..981395 (+) 840 WP_013714032.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44473.13 Da        Isoelectric Point: 9.7208

>NTDB_id=40394 MDS_RS04445 WP_013714029.1 975601..976821(-) (pilC) [Ectopseudomonas mendocina NK-01]
MAEKALKTSVFAWEGTDRKGAKIKGEISGQSPALVKAQLRKQGINPLKVRKKAISLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIAEGFDNPNMRKLIDEIKQEVAAGNSFASSLRKKPEFFDDLYCNLVDSGEQSGALETLLDRIATYKEKT
EALKAKIKKAMTYPIAVIVVAVIVTAILLIKVVPQFESVFEGFGAQLPAFTMVVVNLSRWLQEWWFLVLFAIFISFYTFS
QTYKRSEKFRDSLDRALLKAPIVGDILYKAVVARFARTLATTFAAGVPLVDALDSVAGATGNVVFRNAVNRIKNDVSSGS
QLNFSMRNTGIFPTMAVQMTAIGEEAGSLDEMLDKTASYYEAEVDNAVDNLTTLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=40394 MDS_RS04445 WP_013714029.1 975601..976821(-) (pilC) [Ectopseudomonas mendocina NK-01]
ATGGCGGAAAAAGCACTGAAAACCAGCGTATTCGCCTGGGAAGGTACCGACAGAAAAGGCGCCAAGATCAAAGGAGAGAT
CTCTGGCCAATCTCCCGCACTGGTGAAAGCCCAACTGCGTAAGCAAGGCATCAACCCGCTGAAGGTCCGCAAAAAAGCGA
TTTCCCTCTTTGGTGCCGGGAAGAAAATCAAGCCGATGGACATCGCCCTGTTCACCCGGCAAATGGCGACCATGATGAAA
GCTGGGGTCCCGCTACTGCAGTCCTTTGACATCATCGCCGAGGGTTTCGACAACCCGAACATGCGCAAGTTGATCGACGA
GATCAAGCAAGAAGTGGCGGCAGGTAACAGTTTCGCCTCATCACTGCGCAAGAAGCCTGAGTTTTTCGACGATTTGTACT
GCAACCTGGTGGATTCGGGCGAGCAGTCCGGTGCGCTGGAAACACTGCTCGACAGGATTGCTACCTACAAGGAAAAAACC
GAAGCGCTAAAAGCAAAAATCAAGAAAGCCATGACCTACCCCATCGCGGTAATCGTGGTTGCCGTTATCGTGACTGCGAT
TCTGCTGATCAAGGTAGTACCTCAATTCGAGAGCGTATTCGAAGGTTTTGGCGCCCAGCTACCAGCATTCACCATGGTCG
TGGTCAATCTTTCCAGGTGGCTGCAGGAGTGGTGGTTCCTGGTGCTATTCGCGATCTTCATTTCGTTCTATACCTTCAGC
CAAACCTATAAACGTTCGGAGAAATTTCGCGACTCTCTGGACAGAGCCTTACTCAAGGCTCCCATCGTGGGTGACATTCT
TTATAAAGCGGTTGTAGCGCGATTCGCCCGCACCCTGGCAACGACCTTTGCGGCAGGTGTCCCGCTTGTTGACGCTCTGG
ACTCTGTGGCGGGCGCTACCGGTAACGTGGTGTTTCGCAACGCGGTCAACCGAATCAAGAACGATGTTTCATCAGGTTCA
CAGCTGAACTTCTCCATGCGCAATACCGGCATTTTCCCAACCATGGCAGTCCAGATGACCGCCATCGGCGAAGAAGCAGG
CTCGCTGGATGAAATGCTGGACAAGACTGCCAGCTACTATGAAGCCGAAGTGGATAACGCCGTAGATAACCTTACTACGC
TGATGGAGCCACTGATTATGTCCGTGCTGGGCGTACTGGTTGGCGGCCTGATCATCGCCATGTACCTACCCATCTTCCAA
CTTGGTTCGGTGGTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379IZL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.494

99.754

0.803
  pilC Acinetobacter baylyi ADP1

61.084

100

0.611
  pilC Acinetobacter baumannii D1279779

60.988

99.754

0.608
  pilC Legionella pneumophila strain ERS1305867

53.535

97.537

0.522
  pilG Neisseria gonorrhoeae MS11

44.776

99.015

0.443
  pilG Neisseria meningitidis 44/76-A

44.776

99.015

0.443
  pilC Vibrio cholerae strain A1552

42.118

100

0.421
  pilC Vibrio campbellii strain DS40M4

41.75

98.522

0.411

Multiple sequence alignment