Detailed information NTG0040393

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MDS_RS04440
• Genome accession: NC_015410
• Coordinates: 974731..975597 (-)
• Length: 288 a.a.
• NCBI ID: WP_085982453.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina NK-01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 969731..980597

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MDS_RS04405 (MDS_0878)	-	970891..971064 (-)	174	WP_013714021.1	DUF3094 family protein	-
MDS_RS04410 (MDS_0879)	-	971272..971826 (-)	555	WP_013714022.1	MOSC domain-containing protein	-
MDS_RS04415 (MDS_0880)	-	971823..972452 (-)	630	WP_013714023.1	DUF1780 domain-containing protein	-
MDS_RS04420 (MDS_0881)	-	972661..973350 (+)	690	WP_013714024.1	energy-coupling factor ABC transporter permease	-
MDS_RS04425 (MDS_0882)	-	973456..973671 (+)	216	WP_013714025.1	hypothetical protein	-
MDS_RS04430 (MDS_0883)	yacG	973743..973937 (-)	195	WP_013714026.1	DNA gyrase inhibitor YacG	-
MDS_RS04435 (MDS_0884)	coaE	973934..974542 (-)	609	WP_013714027.1	dephospho-CoA kinase	-
MDS_RS04440 (MDS_0885)	pilD	974731..975597 (-)	867	WP_085982453.1	A24 family peptidase	Machinery gene
MDS_RS04445 (MDS_0886)	pilC	975601..976821 (-)	1221	WP_013714029.1	type II secretion system F family protein	Machinery gene
MDS_RS04450 (MDS_0887)	pilB	976825..978525 (-)	1701	WP_041769664.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MDS_RS26250 (MDS_0888)	-	978753..978938 (+)	186	WP_003242736.1	type IV pilin protein	-
MDS_RS25670	-	978950..979408 (+)	459	WP_147285547.1	hypothetical protein	-
MDS_RS26255	pilA	979497..979676 (+)	180	WP_041769344.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
MDS_RS04465 (MDS_0889)	-	979849..980553 (+)	705	WP_115299238.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31792.68 Da
• Isoelectric Point: 5.6551

>NTDB_id=40393 MDS_RS04440 WP_085982453.1 974731..975597(-) (pilD) [Ectopseudomonas mendocina NK-01]
MALDFMADNLSAFVFVCVVFGLLIGSFLNVVVYRLPLMLQRDWQSQAREVLEQPAAPTTETFNLILPNSSCPNCGHEIRP
WENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSAYVAWHFGFTWQAGAMLVLAWGLLAMSLIDADHQILPDVLVL
PLLWLGLIVNYFGLFTSLEDALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVGA
VLGVIMLRMRGSDSGTPIPFGPYLAIAGWIALLWGDVITGSYLKFAGF

Nucleotide

• Length: 867 bp

>NTDB_id=40393 MDS_RS04440 WP_085982453.1 974731..975597(-) (pilD) [Ectopseudomonas mendocina NK-01]
ATGGCGCTCGACTTTATGGCCGACAATCTGTCGGCCTTTGTTTTTGTCTGTGTAGTTTTTGGCCTGCTAATCGGCAGTTT
CCTAAACGTCGTGGTCTACCGCCTACCACTGATGCTTCAACGCGACTGGCAAAGCCAGGCACGTGAAGTATTGGAACAGC
CAGCGGCGCCGACAACAGAAACTTTCAACCTGATCCTCCCCAACTCCTCATGCCCCAACTGCGGCCATGAGATCCGCCCT
TGGGAAAACATTCCGGTGATCAGCTACCTGTTCCTGCGCGGCAAGTGCTCGAACTGCAAGACGCCTATCAGCAAGCGCTA
CCCTATCGTAGAACTGGCCTGCGGGCTGCTTTCCGCTTACGTCGCCTGGCATTTCGGTTTCACCTGGCAGGCCGGAGCGA
TGCTGGTACTGGCCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGATTCTGCCGGATGTATTGGTGCTT
CCGTTGCTGTGGCTGGGCTTGATCGTCAATTACTTCGGGTTGTTCACCAGCCTGGAAGATGCTCTGTGGGGAGCGATTGC
CGGTTATCTGAGCCTGTGGTCGGTGTATTGGCTGTTCAAGCTGATCACCGGCAAGGAAGGCATGGGTTATGGCGACTTCA
AGCTGCTCGCCATGCTCGGCGCCTGGGGTGGCTGGCAGATTCTGCCGCTGACCATTCTGCTGTCGTCGCTGGTCGGGGCG
GTGCTGGGAGTAATCATGCTGCGCATGCGTGGCAGTGATTCCGGCACGCCGATTCCCTTCGGCCCTTATCTGGCGATTGC
CGGGTGGATCGCCCTGCTATGGGGCGATGTGATTACCGGCAGCTATCTGAAGTTTGCTGGGTTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	53.025	97.569	0.517
pilD	Acinetobacter nosocomialis M2	52.669	97.569	0.514
pilD	Vibrio cholerae strain A1552	53.285	95.139	0.507
pilD	Vibrio campbellii strain DS40M4	52.747	94.792	0.5
pilD	Neisseria gonorrhoeae MS11	49.425	90.625	0.448