Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   HW370_RS21430 Genome accession   NZ_CP058222
Coordinates   4405822..4407204 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain CFT073     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4400822..4412204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW370_RS21410 (HW370_21220) ettA 4402136..4403803 (+) 1668 WP_000046754.1 energy-dependent translational throttle protein EttA -
  HW370_RS21415 (HW370_21225) nadS 4403859..4404143 (-) 285 WP_000007436.1 NadS family protein -
  HW370_RS21420 (HW370_21230) - 4404145..4404477 (-) 333 WP_001298496.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HW370_RS21425 (HW370_21235) nadR 4404569..4405801 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  HW370_RS21430 (HW370_21240) radA/sms 4405822..4407204 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  HW370_RS21435 (HW370_21245) serB 4407253..4408221 (-) 969 WP_001132964.1 phosphoserine phosphatase -
  HW370_RS21440 (HW370_21250) ytjB 4408327..4408971 (+) 645 WP_000124608.1 YtjB family periplasmic protein -
  HW370_RS21445 (HW370_21255) lplA 4408999..4410015 (+) 1017 WP_000105836.1 lipoate--protein ligase LplA -
  HW370_RS21450 (HW370_21260) yjjJ 4410016..4411347 (-) 1332 WP_001293111.1 type II toxin-antitoxin system HipA family toxin YjjJ -
  HW370_RS21455 (HW370_21265) tnpA 4411559..4412017 (-) 459 WP_000526115.1 IS200/IS605-like element IS200C family transposase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=403886 HW370_RS21430 WP_001029698.1 4405822..4407204(-) (radA/sms) [Escherichia coli strain CFT073]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=403886 HW370_RS21430 WP_001029698.1 4405822..4407204(-) (radA/sms) [Escherichia coli strain CFT073]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTCTCCGACATCAGCCTTGAGGAGCTGCCGCGTTTTTCTACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGCTGTTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCAGTCGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGTATCTCAGAAGCGGCGAAACATGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43

Multiple sequence alignment