Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HW370_RS20775 Genome accession   NZ_CP058222
Coordinates   4268380..4268820 (+) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli strain CFT073     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4263380..4273820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW370_RS20755 (HW370_20570) aroP 4264380..4265750 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  HW370_RS20760 (HW370_20575) ampE 4265793..4266647 (-) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  HW370_RS20765 (HW370_20580) ampD 4266644..4267195 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HW370_RS20770 (HW370_20585) nadC 4267283..4268176 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HW370_RS20775 (HW370_20590) pilA 4268380..4268820 (+) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  HW370_RS20780 (HW370_20595) pilB 4268830..4270215 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  HW370_RS20785 (HW370_20600) hofC 4270205..4271407 (+) 1203 WP_000157242.1 protein transport protein HofC -
  HW370_RS20790 (HW370_20605) guaC 4271442..4272485 (-) 1044 WP_001217338.1 GMP reductase -
  HW370_RS20795 (HW370_20610) - 4272641..4272685 (-) 45 WP_120795372.1 protein YacM -
  HW370_RS20800 (HW370_20615) coaE 4272710..4273330 (+) 621 WP_001269525.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=403883 HW370_RS20775 WP_000360915.1 4268380..4268820(+) (pilA) [Escherichia coli strain CFT073]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=403883 HW370_RS20775 WP_000360915.1 4268380..4268820(+) (pilA) [Escherichia coli strain CFT073]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363