Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GK020_RS02845 Genome accession   NZ_CP046392
Coordinates   603465..604262 (+) Length   265 a.a.
NCBI ID   WP_156188783.1    Uniprot ID   -
Organism   Acinetobacter indicus strain WMB-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598465..609262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK020_RS02815 - 598781..599200 (+) 420 WP_005182377.1 hypothetical protein -
  GK020_RS02820 - 599368..600651 (+) 1284 WP_156188781.1 glycosyltransferase family 4 protein -
  GK020_RS02825 - 600684..601235 (+) 552 WP_156188782.1 phosphatase PAP2 family protein -
  GK020_RS02830 iscX 601330..601530 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  GK020_RS02835 ndk 601640..602071 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  GK020_RS02840 rlmN 602194..603429 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GK020_RS02845 pilF 603465..604262 (+) 798 WP_156188783.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GK020_RS02850 - 604253..605041 (+) 789 WP_005182393.1 helix-turn-helix domain-containing protein -
  GK020_RS02855 ispG 605064..606185 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GK020_RS02860 hisS 606189..607484 (+) 1296 WP_005182398.1 histidine--tRNA ligase -
  GK020_RS02865 - 607515..608219 (+) 705 WP_156188784.1 YfgM family protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29974.90 Da        Isoelectric Point: 8.3587

>NTDB_id=403793 GK020_RS02845 WP_156188783.1 603465..604262(+) (pilF) [Acinetobacter indicus strain WMB-7]
MYKFTTISTLCVAVWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=403793 GK020_RS02845 WP_156188783.1 603465..604262(+) (pilF) [Acinetobacter indicus strain WMB-7]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGTATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCGCTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTTAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

71.747

100

0.728

Multiple sequence alignment