Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HW372_RS19575 Genome accession   NZ_CP058217
Coordinates   4084471..4084911 (+) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli Nissle 1917     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4079471..4089911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW372_RS19555 (HW372_19395) aroP 4080471..4081841 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  HW372_RS19560 (HW372_19400) ampE 4081884..4082738 (-) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  HW372_RS19565 (HW372_19405) ampD 4082735..4083286 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HW372_RS19570 (HW372_19410) nadC 4083374..4084267 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HW372_RS19575 (HW372_19415) pilA 4084471..4084911 (+) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  HW372_RS19580 (HW372_19420) pilB 4084921..4086306 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  HW372_RS19585 (HW372_19425) hofC 4086296..4087498 (+) 1203 WP_000157242.1 protein transport protein HofC -
  HW372_RS19590 (HW372_19430) guaC 4087533..4088576 (-) 1044 WP_001217338.1 GMP reductase -
  HW372_RS19595 (HW372_19435) - 4088732..4088776 (-) 45 WP_120795372.1 protein YacM -
  HW372_RS19600 (HW372_19440) coaE 4088801..4089421 (+) 621 WP_001269525.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=403707 HW372_RS19575 WP_000360915.1 4084471..4084911(+) (pilA) [Escherichia coli Nissle 1917]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=403707 HW372_RS19575 WP_000360915.1 4084471..4084911(+) (pilA) [Escherichia coli Nissle 1917]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment