Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   HUS69_RS00620 Genome accession   NZ_CP056073
Coordinates   127866..128534 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EC50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122866..133534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUS69_RS00590 (HUS69_00595) yicC 122885..123748 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  HUS69_RS00595 (HUS69_00600) rph 123875..124591 (+) 717 WP_001247089.1 ribonuclease PH -
  HUS69_RS00600 (HUS69_00605) pyrE 124657..125298 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  HUS69_RS00605 (HUS69_00610) slmA 125335..125931 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  HUS69_RS00610 (HUS69_00615) dut 126038..126496 (-) 459 WP_000976070.1 dUTP diphosphatase -
  HUS69_RS00615 (HUS69_00620) coaBC 126474..127694 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  HUS69_RS00620 (HUS69_00625) radC 127866..128534 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  HUS69_RS00625 (HUS69_00630) rpmB 128751..128987 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  HUS69_RS00630 (HUS69_00635) rpmG 129008..129175 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  HUS69_RS00635 (HUS69_00640) mutM 129273..130082 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  HUS69_RS00640 (HUS69_00645) coaD 130121..130600 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  HUS69_RS00645 (HUS69_00650) waaA 130608..131885 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  HUS69_RS00650 (HUS69_00655) rfaQ 132291..133361 (+) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=403219 HUS69_RS00620 WP_001297375.1 127866..128534(+) (radC) [Escherichia coli strain EC50]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=403219 HUS69_RS00620 WP_001297375.1 127866..128534(+) (radC) [Escherichia coli strain EC50]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment