Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GLO74_RS10660 Genome accession   NZ_CP046354
Coordinates   2086336..2087697 (-) Length   453 a.a.
NCBI ID   WP_075213698.1    Uniprot ID   A0A7X2XL61
Organism   Streptococcus pneumoniae strain 310     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2081336..2092697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLO74_RS10875 - 2082421..2083303 (+) 883 Protein_2075 Rpn family recombination-promoting nuclease/putative transposase -
  GLO74_RS10640 (GLO74_10845) - 2083430..2083711 (+) 282 Protein_2076 transposase family protein -
  GLO74_RS10645 (GLO74_10850) - 2083845..2084813 (-) 969 WP_000010157.1 ribose-phosphate diphosphokinase -
  GLO74_RS10650 (GLO74_10855) - 2084958..2085741 (-) 784 Protein_2078 PrsW family glutamic-type intramembrane protease -
  GLO74_RS10655 (GLO74_10860) - 2085766..2086263 (-) 498 WP_001809263.1 beta-class carbonic anhydrase -
  GLO74_RS10660 (GLO74_10865) radA 2086336..2087697 (-) 1362 WP_075213698.1 DNA repair protein RadA Machinery gene
  GLO74_RS10665 (GLO74_10870) - 2087711..2088226 (-) 516 WP_000691236.1 histidine phosphatase family protein -
  GLO74_RS10670 (GLO74_10875) - 2088228..2088671 (-) 444 WP_000701992.1 dUTP diphosphatase -
  GLO74_RS10675 - 2088976..2089125 (+) 150 WP_001030863.1 hypothetical protein -
  GLO74_RS10680 (GLO74_10880) - 2089267..2089446 (+) 180 WP_001209433.1 hypothetical protein -
  GLO74_RS10685 (GLO74_10885) - 2089666..2090046 (-) 381 Protein_2085 autolysin -
  GLO74_RS10690 (GLO74_10890) - 2090051..2090218 (+) 168 WP_000024181.1 YjzC family protein -
  GLO74_RS10695 (GLO74_10895) - 2090373..2090576 (-) 204 WP_001247549.1 hypothetical protein -
  GLO74_RS10700 (GLO74_10900) - 2090599..2090790 (-) 192 WP_001112859.1 DNA-binding protein -
  GLO74_RS10705 (GLO74_10905) - 2091362..2091730 (+) 369 WP_000464160.1 helix-turn-helix domain-containing protein -
  GLO74_RS10710 (GLO74_10910) - 2091730..2091963 (+) 234 WP_000156419.1 hypothetical protein -
  GLO74_RS10715 (GLO74_10915) - 2091963..2092226 (+) 264 WP_000285962.1 type II toxin-antitoxin system RelE family toxin -
  GLO74_RS10720 (GLO74_10920) - 2092239..2092619 (+) 381 WP_000170931.1 ImmA/IrrE family metallo-endopeptidase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.91 Da        Isoelectric Point: 6.1868

>NTDB_id=402989 GLO74_RS10660 WP_075213698.1 2086336..2087697(-) (radA) [Streptococcus pneumoniae strain 310]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=402989 GLO74_RS10660 WP_075213698.1 2086336..2087697(-) (radA) [Streptococcus pneumoniae strain 310]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTCAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X2XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment