Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUU83_RS03385 Genome accession   NZ_CP055251
Coordinates   718793..719233 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain AH01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713793..724233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU83_RS03360 (HUU83_03360) coaE 714283..714903 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  HUU83_RS03365 (HUU83_03365) - 714928..714972 (+) 45 WP_120795372.1 protein YacM -
  HUU83_RS03370 (HUU83_03370) guaC 715128..716171 (+) 1044 WP_001217338.1 GMP reductase -
  HUU83_RS03375 (HUU83_03375) hofC 716206..717408 (-) 1203 WP_000157234.1 protein transport protein HofC -
  HUU83_RS03380 (HUU83_03380) pilB 717398..718783 (-) 1386 WP_001470984.1 type II secretion system protein GspE Machinery gene
  HUU83_RS03385 (HUU83_03385) pilA 718793..719233 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  HUU83_RS03390 (HUU83_03390) nadC 719436..720329 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU83_RS03395 (HUU83_03395) ampD 720417..720968 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUU83_RS03400 (HUU83_03400) ampE 720965..721819 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  HUU83_RS03405 (HUU83_03405) aroP 721862..723235 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=402898 HUU83_RS03385 WP_000360895.1 718793..719233(-) (pilA) [Escherichia coli strain AH01]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=402898 HUU83_RS03385 WP_000360895.1 718793..719233(-) (pilA) [Escherichia coli strain AH01]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363