Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSEBR_RS02325 Genome accession   NC_015379
Coordinates   523406..525496 (+) Length   696 a.a.
NCBI ID   WP_013692093.1    Uniprot ID   F2KDE9
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518406..530496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS02305 (PSEBR_a422) pilM 520618..521682 (+) 1065 WP_013692092.1 pilus assembly protein PilM Machinery gene
  PSEBR_RS02310 (PSEBR_a423) - 521682..522248 (+) 567 WP_003196975.1 PilN domain-containing protein -
  PSEBR_RS02315 (PSEBR_a424) pilO 522245..522868 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  PSEBR_RS02320 (PSEBR_a425) - 522865..523392 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  PSEBR_RS02325 (PSEBR_a426) pilQ 523406..525496 (+) 2091 WP_013692093.1 type IV pilus secretin PilQ Machinery gene
  PSEBR_RS02330 (PSEBR_a427) aroK 525501..526019 (+) 519 WP_003196983.1 shikimate kinase AroK -
  PSEBR_RS02335 (PSEBR_a428) aroB 526168..527268 (+) 1101 WP_003196985.1 3-dehydroquinate synthase -
  PSEBR_RS02340 (PSEBR_a429) - 527278..528858 (+) 1581 WP_003196987.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75130.19 Da        Isoelectric Point: 5.2961

>NTDB_id=40262 PSEBR_RS02325 WP_013692093.1 523406..525496(+) (pilQ) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MNRFFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPSPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAALKSTAPKNSTTQAPRPAVASAAPARASAPVGK
AIRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIE
PSGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKATDIAKLFQSVTS
AEAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWSSS
GVTTGDSTTVGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=40262 PSEBR_RS02325 WP_013692093.1 523406..525496(+) (pilQ) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTCGGGGCCAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCTCCTTCGACGGGCCGCCGCCGTCACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACTGTGGTCGAGGCTGGGGATCGTACTCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATGACACCCGGATCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCCTGAAGAGCACTGCCC
CGAAGAACTCCACCACCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAG
GCGATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGACCTGTCTGATCCGTCCATTGC
CCCGGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCC
TGGACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAA
CCCAGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGA
CCTGCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTT
CGGTCCTGCAACTGATTGCCGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGG
TTGCAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGT
GCTGTTGGTGGCTCCGGCCGATGAAATTGCCGCTCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACTTGG
CGCCGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGACGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGA
CCGCCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCG
AAGCCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGGTTCAAAACAAGGGCAACTGGAGCTCCTCG
GGGGTTACCACGGGCGATTCCACCACCGTTGGCACGCCGGGTAGCACCAGCACCAACCAGCCGTTCGTCGACCTGGGCGC
CTCGGGCAATACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAGA
AAACCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACG
GAAATTCCTTATCAGGAAGCCAGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGAC
CCCGCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGA
ATGATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2KDE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.398

100

0.757
  pilQ Acinetobacter baumannii strain A118

38.71

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.71

100

0.414
  comQ Acinetobacter baylyi ADP1

40.805

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

38.201

97.414

0.372
  pilQ Legionella pneumophila strain Lp02

37.794

97.701

0.369

Multiple sequence alignment