Detailed information NTG0040261

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PSEBR_RS02305
• Genome accession: NC_015379
• Coordinates: 520618..521682 (+)
• Length: 354 a.a.
• NCBI ID: WP_013692092.1
• Uniprot ID: F2KDE5
• Organism: Pseudomonas brassicacearum subsp. brassicacearum NFM421
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 515618..526682

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSEBR_RS02295 (PSEBR_a420)	-	516451..517719 (+)	1269	WP_003196970.1	malic enzyme-like NAD(P)-binding protein	-
PSEBR_RS02300 (PSEBR_a421)	-	517965..520409 (-)	2445	WP_371916716.1	penicillin-binding protein 1A	-
PSEBR_RS02305 (PSEBR_a422)	pilM	520618..521682 (+)	1065	WP_013692092.1	pilus assembly protein PilM	Machinery gene
PSEBR_RS02310 (PSEBR_a423)	-	521682..522248 (+)	567	WP_003196975.1	PilN domain-containing protein	-
PSEBR_RS02315 (PSEBR_a424)	pilO	522245..522868 (+)	624	WP_003196977.1	type 4a pilus biogenesis protein PilO	-
PSEBR_RS02320 (PSEBR_a425)	-	522865..523392 (+)	528	WP_003196979.1	pilus assembly protein PilP	-
PSEBR_RS02325 (PSEBR_a426)	pilQ	523406..525496 (+)	2091	WP_013692093.1	type IV pilus secretin PilQ	Machinery gene
PSEBR_RS02330 (PSEBR_a427)	aroK	525501..526019 (+)	519	WP_003196983.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38111.67 Da
• Isoelectric Point: 4.9070

>NTDB_id=40261 PSEBR_RS02305 WP_013692092.1 520618..521682(+) (pilM) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=40261 PSEBR_RS02305 WP_013692092.1 520618..521682(+) (pilM) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCTGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCGGTGGCGGGTTCAGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGATGGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCATTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTTGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATCCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCTGGCCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGACTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
CCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAATGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: F2KDE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	55.085	100	0.551
pilM	Legionella pneumophila strain ERS1305867	47.458	100	0.475